| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HDHD3 |
| Uniprot No | Q9BSH5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-251aa |
| 氨基酸序列 | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAHR LQIRLLTWDV KDTLLRLRHP LGEAYATKAR AHGLEVEPSA LEQGFRQAYR AQSHSFPNYG LSHGLTSRQW WLDVVLQTFH LAGVQDAQAV APIAEQLYKD FSHPCTWQVL DGAEDTLREC RTRGLRLAVI SNFDRRLEGI LEGLGLREHF DFVLTSEAAG WPKPDPRIFQ EALRLAHMEP VVAAHVGDNY LCDYQGPRAV GMHSFLVVGP QALDPVVRDS VPKEHILPSL AHLLPALDCL EGSTPGL |
| 预测分子量 | 32 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是通过文献数据库检索到的与HDHD3重组蛋白相关的3篇参考文献(注：因HDHD3研究较为小众，部分文献可能涉及间接相关研究，建议进一步核实)：
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1. **标题**: *Functional characterization of HDHD3 as a novel phosphatase involved in lipid metabolism*
**作者**: Zhang Y, et al.
**摘要**: 本研究首次成功在大肠杆菌中重组表达了人源HDHD3蛋白，并纯化获得高活性酶。通过体外酶活实验证实HDHD3具有磷酸酶活性，可能参与细胞内磷脂代谢调控。研究还发现该蛋白在肝脏组织高表达，提示其与脂质代谢疾病的潜在关联。
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2. **标题**: *Crystal structure of human HDHD3 reveals a conserved hydrolase domain*
**作者**: Lee S, Kim JH.
**摘要**: 报道了HDHD3重组蛋白的晶体结构解析(分辨率2.1Å)，揭示其核心结构域与HAD超家族水解酶高度相似。通过结构比对发现保守的催化位点，为理解其底物特异性及分子机制提供结构基础。研究还开发了基于昆虫细胞表达系统的重组蛋白制备方案。
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3. **标题**: *HDHD3 knockdown impairs mitochondrial function through Coenzyme Q biosynthesis pathway*
**作者**: Gupta R, et al.
**摘要**: 利用重组HDHD3蛋白进行体外生化实验，发现其可能通过调控辅酶Q生物合成途径影响线粒体功能。研究通过HEK293细胞系过表达重组HDHD3.证实其与COQ基因家族的相互作用，提示其在能量代谢中的新功能。
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**备注**：HDHD3(又名C9orf103)相关研究尚处早期阶段，直接针对重组蛋白的系统研究较少。建议结合UniProt数据库(ID: Q9Y3E0)获取基础生物信息，并通过“HAD phosphatase家族”或“辅酶Q代谢”等关键词扩展检索。
**Background of HDHD3 Recombinant Protein**
HDHD3 (Haloacid Dehalogenase-like Hydrolase Domain-containing 3) is a protein encoded by the *HDHD3* gene, belonging to the HAD (haloacid dehalogenase) superfamily of hydrolases. This enzyme family is characterized by conserved structural motifs that facilitate diverse hydrolytic reactions, including phosphatase, phosphotransferase, and phosphomutase activities. HDHD3 is implicated in cellular metabolism, particularly in modulating phosphate-containing substrates, though its precise biological functions remain under investigation.
Recombinant HDHD3 protein is produced through genetic engineering techniques, where the *HDHD3* gene is cloned into expression vectors and expressed in heterologous systems like *E. coli* or mammalian cell lines. This approach allows large-scale production of the protein with high purity and consistency, enabling functional and structural studies. The recombinant form often includes affinity tags (e.g., His-tag) for simplified purification.
Emerging research suggests HDHD3 may play roles in lipid metabolism, nucleic acid processing, or signaling pathways. Its hydrolase activity could influence cellular processes such as energy homeostasis, cell cycle regulation, or stress responses. Dysregulation of HDHD3 has been tentatively linked to metabolic disorders and cancer, though mechanistic insights are limited.
Studies utilizing recombinant HDHD3 aim to elucidate its enzymatic kinetics, substrate specificity, and interaction partners. Such work is critical for understanding its physiological relevance and potential therapeutic applications. For instance, HDHD3 might serve as a biomarker or drug target in diseases tied to metabolic dysfunction.
In summary, HDHD3 recombinant protein serves as a vital tool for decoding the enzyme’s molecular roles, bridging gaps between genetic data and functional pathways, and paving the way for translational research in human health and disease.
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