| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | Aldh2 |
| Uniprot No | P05091 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-517aa |
| 氨基酸序列 | MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLN RLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAG TVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S |
| 预测分子量 | 55 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ALDH2重组蛋白的3篇参考文献的简要概括(内容基于领域内典型研究方向，非真实文献)：
1. **《高效表达与纯化人源ALDH2重组蛋白及其酶活性分析》**
- 作者：Zhang et al.
- 摘要：研究通过大肠杆菌表达系统成功重组表达了人源ALDH2蛋白，并优化纯化条件获得高纯度蛋白。酶动力学实验表明，重组ALDH2对乙醛的催化效率与天然蛋白一致，证实其功能完整性，为后续药物筛选提供基础。
2. **《ALDH2 rs671突变体重组蛋白的构效关系研究》**
- 作者：Wang et al.
- 摘要：针对亚洲人群常见的ALDH2 rs671突变(Glu504Lys)，构建并表达突变型重组蛋白。结构分析显示，突变导致酶活性中心构象改变，乙醛代谢能力显著下降，解释了酒精不耐受的分子机制。
3. **《ALDH2重组蛋白在缺血再灌注损伤中的保护作用》**
- 作者：Chen et al.
- 摘要：利用昆虫细胞表达系统制备ALDH2重组蛋白，通过动物模型验证其通过清除4-HNE等毒性醛类，减轻心脏缺血再灌注损伤的效果，提示其作为治疗药物的潜力。
(注：以上为模拟摘要，实际文献需通过PubMed、Google Scholar等平台检索关键词“ALDH2 recombinant protein”获取。)
Aldehyde dehydrogenase 2 (ALDH2) is a mitochondrial enzyme critical in detoxifying reactive aldehydes, particularly acetaldehyde—a toxic byproduct of ethanol metabolism. It also plays a role in metabolizing lipid peroxidation-derived aldehydes, such as 4-hydroxynonenal, which are linked to oxidative stress and cellular damage. ALDH2 is highly expressed in the liver, heart, and brain, and its dysfunction is associated with various diseases, including cardiovascular disorders, neurodegenerative conditions, and alcohol-related pathologies. A common genetic variant (Glu504Lys, rs671), prevalent in East Asian populations, reduces ALDH2 activity by ~90%, leading to "alcohol flush syndrome" and increased susceptibility to conditions like esophageal cancer and myocardial infarction.
Recombinant ALDH2 protein is engineered using expression systems like *E. coli*, yeast, or mammalian cells to produce purified, functional enzyme for research and therapeutic applications. Its production enables detailed structural studies (e.g., crystallography) to elucidate substrate binding and catalytic mechanisms. Researchers use recombinant ALDH2 to screen activators or inhibitors, aiming to develop drugs targeting ALDH2-deficient conditions or alcohol use disorders. For instance, small-molecule activators (e.g., Alda-1) have been explored to enhance mutant ALDH2 activity, potentially mitigating ischemia-reperfusion injury or alcohol toxicity. Additionally, recombinant ALDH2 serves as a biomarker in studies linking aldehyde accumulation to age-related diseases. Challenges include preserving post-translational modifications and mitochondrial localization in vitro, which are essential for its native function. Overall, recombinant ALDH2 is a vital tool for advancing therapeutic strategies against aldehyde-mediated toxicity and genetic susceptibility syndromes.
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