| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HDHD2 |
| Uniprot No | Q9H0R4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-259aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMAACRALKAVLVDLSGTLHIEDAAVPGAQE ALKRLRGASVIIRFVTNTTKESKQDLLERLRKLEFDISEDEIFTSLTAAR SLLERKQVRPMLLVDDRALPDFKGIQTSDPNAVVMGLAPEHFHYQILNQA FRLLLDGAPLIAIHKARYYKRKDGLALGPGPFVTALEYATDTKATVVGKP EKTFFLEALRGTGCEPEEAVMIGDDCRDDVGGAQDVGMLGILVKTGKYRA SDEEKINPPPYLTCESFPHAVDHILQHLL |
| 预测分子量 | 31 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HDHD2重组蛋白的3篇参考文献的简要信息(注：HDHD2相关研究较少，以下为模拟示例，实际文献需根据具体数据库检索确认)：
1. **文献名称**: "Cloning, Expression, and Functional Characterization of Human HDHD2 as a Novel Phosphatase"
**作者**: Smith A, et al.
**摘要**: 该研究成功克隆并表达了人源HDHD2重组蛋白，利用大肠杆菌表达系统纯化获得高纯度蛋白。功能实验表明HDHD2具有磷酸酶活性，可能参与细胞内磷酸代谢调控。
2. **文献名称**: "Structural Insights into HDHD2 Protein: Crystallographic Analysis of a Recombinant Hydrolase"
**作者**: Lee B, et al.
**摘要**: 通过X射线晶体学解析了HDHD2重组蛋白的三维结构，揭示了其催化结构域特征，并发现其与细菌脱卤酶具有结构同源性，提示其在底物水解中的作用机制。
3. **文献名称**: "HDHD2 Knockdown and Recombinant Protein Rescue in Cellular Models of Neurodevelopmental Disorders"
**作者**: Chen X, et al.
**摘要**: 研究利用HDHD2重组蛋白在神经细胞模型中进行功能回补实验，发现HDHD2缺失导致线粒体功能异常，重组蛋白可部分恢复表型，提示其在神经发育中的潜在作用。
**备注**：若实际文献不足，建议扩展检索关键词(如基因别名“C14orf160”或功能关键词)，或关注相关蛋白家族(如HAD超家族)的研究。
**Background of HDHD2 Recombinant Protein**
HDHD2 (Haloacid Dehalogenase-like Hydrolase Domain-containing protein 2) is a member of the haloacid dehalogenase (HAD)-like hydrolase superfamily, characterized by conserved structural motifs involved in phosphatase, phosphotransferase, or phosphohydrolase activities. This enzyme plays a potential role in cellular metabolism, particularly in regulating phosphate-containing substrates, though its precise biological functions remain under investigation. HDHD2 is encoded by the *HDHD2* gene, which is evolutionarily conserved across eukaryotes, suggesting its fundamental role in cellular processes.
Recombinant HDHD2 protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells*) to produce purified, functional protein for biochemical and cellular studies. Its recombinant form enables researchers to study enzymatic activity, substrate specificity, and interactions with other biomolecules. Structural analyses reveal conserved catalytic domains critical for hydrolyzing phosphoester bonds, implicating HDHD2 in nucleotide metabolism, signal transduction, or detoxification pathways.
Emerging studies link HDHD2 dysregulation to human diseases. For example, altered expression has been observed in certain cancers and neurological disorders, hinting at roles in cell proliferation or neuronal function. Additionally, genetic variants near *HDHD2* loci are associated with metabolic syndromes, though mechanistic insights are limited.
As a research tool, recombinant HDHD2 aids in elucidating its physiological role, screening inhibitors for therapeutic development, or generating antibodies for diagnostic applications. Further studies are needed to map its exact pathways and validate its potential as a biomarker or drug target.
×
