| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HDHD1A |
| Uniprot No | Q08623 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-228aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMAAPPQPVTHLIFDMDGLLLDTERLYSVVF QEICNRYDKKYSWDVKSLVMGKKALEAAQIIIDVLQLPMSKEELVEESQT KLKEVFPTAALMPGAEKLIIHLRKHGIPFALATSSGSASFDMKTSRHKEF FSLFSHIVLGDDPEVQHGKPDPDIFLACAKRFSPPPAMEKCLVFEDAPNG VEAALAAGMQVVMVPDGNLSRDLTTKATLVLNSLQDFQPELFGLPSYE |
| 预测分子量 | 27 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HDHD1A重组蛋白的假设性参考文献示例(注：经检索，HDHD1A相关研究较少，实际文献可能有限，以下为模拟内容供参考)：
---
1. **文献名称**: "Cloning and Expression of Recombinant HDHD1A in E. coli for Functional Characterization"
**作者**: Zhang L, et al.
**摘要**: 本研究成功构建了HDHD1A基因的重组表达载体，并在大肠杆菌中实现了可溶性表达。通过亲和层析纯化获得高纯度蛋白，并初步验证其水解酶活性，为后续结构功能研究奠定基础。
2. **文献名称**: "Structural Insights into HDHD1A by X-ray Crystallography"
**作者**: Smith J, et al.
**摘要**: 利用重组HDHD1A蛋白进行晶体结构解析，揭示了其催化结构域的关键氨基酸残基，推测其可能参与磷酸酶或水解酶类反应，为设计功能抑制剂提供依据。
3. **文献名称**: "HDHD1A Knockdown and Metabolic Pathway Analysis in Hepatocytes"
**作者**: Tanaka K, et al.
**摘要**: 通过siRNA干扰和重组蛋白回补实验，发现HDHD1A可能调节肝细胞中甘油磷脂代谢通路，提示其在脂质稳态中的潜在作用。
---
**注意**：HDHD1A(Haloacid Dehalogenase-like Hydrolase Domain-containing 1A)的研究尚处于早期阶段，实际公开发表文献较少。建议通过 **UniProt(ID: Q96ES6)** 或 **NCBI Gene数据库** 获取基础信息，并结合相关水解酶家族(如HAD超家族)的研究进行扩展分析。如需真实文献，建议使用专业数据库进一步检索。
**Background of HDHD1A Recombinant Protein**
HDHD1A (Haloacid Dehalogenase-like Hydrolase Domain-Containing Protein 1A) is a protein encoded by the *HDHD1A* gene, belonging to the HAD (haloacid dehalogenase)-like hydrolase superfamily. This family is characterized by conserved structural motifs associated with phosphatase, phosphotransferase, or phosphohydrolase activities, often involved in metabolic regulation, signaling, or detoxification processes. HDHD1A contains a conserved hydrolase domain, suggesting potential roles in hydrolyzing phosphoester bonds or modifying phosphorylated substrates, though its precise biological function remains understudied.
Recombinant HDHD1A protein is engineered using heterologous expression systems (e.g., *E. coli*, mammalian cells) to enable functional and structural studies. The protein is typically purified via affinity tags (e.g., His-tag) for experimental applications. Its recombinant form allows researchers to investigate enzymatic activity, substrate specificity, and interactions with other cellular components.
Emerging evidence links HDHD1A to cellular stress responses, nucleotide metabolism, and possibly DNA repair pathways. Dysregulation of hydrolases in this family has been implicated in metabolic disorders, neurodegenerative diseases, and cancer, positioning HDHD1A as a potential biomarker or therapeutic target. However, detailed mechanistic insights into its physiological and pathological roles are limited, necessitating further exploration.
Current research leverages recombinant HDHD1A to develop assays for inhibitor screening, study post-translational modifications, or generate antibodies for cellular localization studies. Its characterization contributes to understanding the HAD superfamily's diversity and evolution, bridging gaps in enzymology and disease biology.
×
