| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AFUA |
| Uniprot No | Q4WVG0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1106aa |
| 氨基酸序列 | MDGDDLIASVYRKIEREKALITAASNMRQSTDNPLVQQRVDANIRDGRKNIAYLEEKMRELQLRQMKQEGASPTDKRLPPNPDGSAPVPPPKDYAPGYSGHEREYGDASGAYPHGGAGTMPSGAPFADPRPFAPVPKARPNYTKLDLIKYDTPYLGPKIQLMLSQLEFKLSVEKQYKAGIEKMVRLYQDEGDRKSRQDAEGRRIESNQKIQLLKQALKRYEDLHVDIESAEAPDDESLSTPNLRKPLTGLLTLRIHAVEDVDHAASSRFSRGPETFVVVKVEDAIKARTKATRTDKWQDEPFNIEIDKANEIELTVYDKSGDRPTPIGMLWVRISDIAEEMRRKKIESEFNASGWVSADKMEHGAAHGRQDAGGAPGSSNRPPSGGHSGGPGQGYAGGAPGGASAGPVLIDSWFALEPVGRIHLTLSFAKQLKDRRPFDIGLNRQGAVRQKKEEVHEKQGHKFVTQQFYNIMRCALCGDFLKYAAGMQCADCKYTCHRKCYPKVVTKCISKANYETDPDEEKINHRIPHRFEGFSNISANWCCHCGYLLPFGRKSAKRCTECGLTCHAQCTHLVPDFCGMSMEAANQILETLIRAKNHNKSASVSSGLSGRTLRPGGPPQAPQDNVALAYPQKPVEGAYGAPQRQPSAEAISAATNTYIPPQSPTAAQRQHIPPRTSSSQSPAAAAAAAAAAATGLRTPQQASDPNRPVQPPPSSHAHYDPAAYASYQQAIPPQAMQKMGAPYGMPQQQQQQAVAPMQQQVAVKEEIPPQQPKVRIGLDHFNFLAVLGKGNFGKVMLAETKSTKKLYAIKVLKKEFIIENDEVESTKSEKRVFLIANKERHPFLLNLHACFQTETRVYFVMEYISGGDLMLHIQRGQFGLKRAQFYAAEVLLALKYFHENGVIYRDLKLDNILLTLDGHIKIADYGLCKENMWYGSTTSTFCGTPEFMAPEILLDKKYGRAVDWWAFGVLIYQMLLQQSPFRGEDEDEIYDAILADEPLYPIHMPRDSVSILQKLLTREPELRLGSGPTDAQEVMSHAFFRNINWDDIYHKRVPPPFLPTISSPTDTSNFDQEFTSVTPVLTPVQSVLSQAMQEEFRGFSYTADFA |
| 预测分子量 | 123 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AFUA(Aspergillus fumigatus)重组蛋白的3篇示例参考文献(内容为虚构示例，仅作格式参考)：
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1. **文献名称**: *Heterologous Expression and Characterization of AFUA_6g03440 Recombinant Protein in Pichia pastoris*
**作者**: Zhang L, Wang Y, et al.
**摘要**: 本研究在毕赤酵母中成功表达了AFUA_6g03440(一种推测的毒力因子重组蛋白)，并通过SDS-PAGE和质谱验证其正确性。纯化后的蛋白在体外抑制宿主免疫细胞活性，表明其可能在曲霉感染中起关键作用。
2. **文献名称**: *Immunodiagnostic Potential of Recombinant AFUA_5g02370 Antigen for Invasive Aspergillosis*
**作者**: Gupta S, Kumar R, et al.
**摘要**: 通过大肠杆菌系统表达并纯化AFUA_5g02370重组蛋白，发现其与侵袭性曲霉病患者血清特异性结合，敏感性达92%。该蛋白被提议作为新型诊断标志物候选。
3. **文献名称**: *Crystal Structure Analysis of AFUA_1g17210 Recombinant Enzyme Reveals Novel Catalytic Mechanisms*
**作者**: Müller J, Schmidt D, et al.
**摘要**: 解析了AFUA_1g17210重组蛋白的晶体结构(分辨率2.1Å)，发现其具有独特的底物结合口袋，并通过定点突变验证了关键活性位点，为抗真菌药物设计提供结构基础。
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注：以上文献信息为模拟生成，实际引用需查询真实数据库(如PubMed、Web of Science)获取权威来源。
**Background of AFUA Recombinant Proteins**
*Aspergillus fumigatus* (AFUA), a ubiquitous saprophytic fungus, is a leading cause of invasive aspergillosis, particularly in immunocompromised individuals. Its pathogenicity is linked to secreted proteins and cell wall components that facilitate host adhesion, immune evasion, and tissue invasion. Recombinant AFUA proteins are engineered versions of these native proteins, produced using heterologous expression systems (e.g., *E. coli*, yeast, or mammalian cells*) to enable standardized and scalable studies.
The development of recombinant AFUA proteins addresses challenges in studying native fungal proteins, which are often difficult to purify in sufficient quantities due to low natural expression or contamination risks. Recombinant technology allows precise control over protein folding, post-translational modifications (when using eukaryotic systems), and antigenic properties, enhancing reproducibility in research.
These proteins are pivotal in diagnostics, vaccine development, and drug discovery. For example, recombinant antigens like Asp f 1. Asp f 3. or galactomannan-related proteins are used in serological assays to detect *A. fumigatus*-specific antibodies or antigens in patient samples. In vaccine research, recombinant proteins serve as immunogens to elicit protective immune responses. Additionally, they facilitate structural and functional studies to unravel host-pathogen interactions, such as how AFUA proteins bind to host receptors or resist antifungal drugs.
Moreover, recombinant AFUA proteins contribute to understanding fungal virulence mechanisms and identifying therapeutic targets. By dissecting their roles in pathogenicity, researchers aim to design inhibitors or monoclonal antibodies to block critical pathways. Overall, recombinant AFUA proteins are indispensable tools in advancing diagnostic, therapeutic, and mechanistic research against aspergillosis, a disease with high mortality rates in vulnerable populations.
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