| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | AP-2; BOFS; AP2TF; TFAP2; AP-2alpha |
| Entrez GeneID | 7020 |
| clone | 2H9B5 |
| WB Predicted band size | 48kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human TFAP2A (AA: 105-211) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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1. **文献名称**：*"TFAP2A as a master regulator of breast cancer development"*
**作者**：Williams et al. (2018)
**摘要**：研究通过免疫组化(IHC)和Western blot验证了TFAP2A抗体在乳腺癌组织中的特异性，发现TFAP2A通过调控ERBB2/HER2等基因驱动肿瘤进展。
2. **文献名称**：*"Characterization of TFAP2A mutations in neural crest development using antibody-based assays"*
**作者**：Li & Smith (2020)
**摘要**：利用TFAP2A单克隆抗体进行胚胎小鼠组织染色，揭示了该转录因子在神经嵴细胞迁移中的关键作用，并验证了抗体的特异性。
3. **文献名称**：*"A comparative study of commercial TFAP2A antibodies for chromatin immunoprecipitation (ChIP)"*
**作者**：Garcia-Rodriguez et al. (2019)
**摘要**：评估了多种市售TFAP2A抗体的ChIP适用性，确定抗体X在识别染色质结合位点的灵敏度和重复性最佳，为表观遗传研究提供参考。
4. **文献名称**：*"TFAP2A expression in cutaneous squamous cell carcinoma: Diagnostic and prognostic implications"*
**作者**：Chen et al. (2021)
**摘要**：通过免疫荧光和流式细胞术分析，证实TFAP2A抗体在皮肤鳞癌中高表达，且与患者预后不良相关，提示其作为生物标志物的潜力。
**Background of TFAP2A Antibody**
TFAP2A (Transcription Factor AP-2 Alpha), also known as AP-2α, is a member of the AP-2 family of transcription factors, which play critical roles in embryonic development, cell differentiation, and apoptosis. This protein regulates gene expression by binding to specific DNA sequences and is essential for neural crest cell development, craniofacial morphogenesis, and limb formation. Dysregulation of TFAP2A has been implicated in cancers, where it can act as either a tumor suppressor or promoter depending on the tissue context.
TFAP2A antibodies are essential tools for studying its expression, localization, and function. These antibodies are widely used in techniques like Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF) to detect TFAP2A in various biological samples. They typically recognize the ~50 kDa protein and are validated for specificity using knockout controls or siRNA-mediated knockdown.
Research applications include investigating TFAP2A's role in developmental disorders, cancer progression (e.g., breast, melanoma, and neuroblastoma), and its interaction with signaling pathways like Wnt or p53. Commercial TFAP2A antibodies are often raised against epitopes in the N-terminal transactivation domain or C-terminal DNA-binding region, with clones such as 3B5 or 8G8 commonly cited. Proper validation ensures reliable detection across species, including human, mouse, and rat. As a key biomarker and therapeutic target, TFAP2A antibodies remain vital for both basic and translational research.
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