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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HDGF |
| Uniprot No | P51858 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-240aa |
| 氨基酸序列 | MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFF GTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGY QSSQKKSCVEEPEPEPEAAEGDGDKKGNAEGSSDEEGKLVIDEPAKEKNE KGALKRRAGDLLEDSPKRPKEAENPEGEEKEAATLEVERPLPMEVEKNST PSEPGSGRGPPQEEEEEEDEEEEATKEDAEAPGIRDHESL |
| 预测分子量 | 53 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HDGF重组蛋白的3篇参考文献示例(注：文献信息为模拟，实际引用需核对原文准确性)：
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1. **文献名称**: *Hepatoma-derived growth factor promotes cell proliferation and invasion via PI3K/AKT signaling in lung cancer*
**作者**: Kishima, Y., et al.
**摘要**: 研究利用重组HDGF蛋白处理肺癌细胞，发现其通过激活PI3K/AKT通路促进细胞增殖和侵袭，并上调cyclin D1和基质金属蛋白酶(MMPs)的表达。
2. **文献名称**: *Recombinant HDGF enhances angiogenesis in hepatocellular carcinoma through upregulating VEGF expression*
**作者**: Zhang, L., et al.
**摘要**: 通过大肠杆菌表达重组HDGF蛋白，证实其能通过ERK1/2通路诱导肝癌细胞分泌血管内皮生长因子(VEGF)，促进肿瘤血管生成。
3. **文献名称**: *Structural characterization of the HATH domain in HDGF and its DNA-binding activity*
**作者**: Sue, S.C., et al.
**摘要**: 利用重组HDGF的HATH结构域进行结构解析，发现该区域对DNA结合及与其他核蛋白(如RNA聚合酶II)的互作至关重要。
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**注**：以上文献信息为示例，实际研究中建议通过PubMed或Google Scholar检索最新文献并核对作者、年份及摘要细节。
Hepatoma-derived growth factor (HDGF) is a secretory protein initially identified in human hepatoma cells. Belonging to the HDGF-related protein (HRP) family, it contains conserved N-terminal PWWP (Pro-Trp-Trp-Pro) and C-terminal growth factor-like domains. The PWWP domain facilitates chromatin binding and nuclear localization, while the C-terminal domain mediates protein-protein interactions and extracellular signaling. Though primarily nuclear, HDGF can be secreted via non-classical pathways to exert paracrine effects.
Functionally, HDGF acts as a mitogen promoting cell proliferation, survival, and angiogenesis through MAPK/ERK and PI3K/AKT pathways. It participates in embryogenesis, tissue repair, and tumor progression, with elevated expression observed in hepatocellular carcinoma, lung cancer, and gastrointestinal malignancies. Its oncogenic properties include stimulating metastasis, chemo-resistance, and vascular endothelial growth factor (VEGF) production. Paradoxically, HDGF also demonstrates protective roles in cardiovascular diseases by enhancing myocardial repair and reducing ischemic injury.
Recombinant HDGF proteins, typically produced in Escherichia coli or mammalian expression systems, retain biological activity for experimental and therapeutic exploration. They enable mechanistic studies of HDGF's dual roles in carcinogenesis and tissue regeneration, serving as tools for developing targeted inhibitors or recombinant therapeutics. Current challenges involve optimizing post-translational modifications in recombinant forms and clarifying context-dependent signaling mechanisms. As a multifunctional regulator bridging developmental biology and disease pathology, HDGF remains a compelling subject for both basic research and translational applications.
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