| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | dotB |
| Uniprot No | Q83B70 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-372aa |
| 氨基酸序列 | MIEKSQRYPNEPSRFEPKHIDDLLIYCHRLGASDITIQTGRAVLAEVYGRLQTLTRRELSNAETSDLLNAIYGPNGTTQIMRGEDIDTHYEIRPNRNERFRYRINGTGCHVDGHEGIQITIRAIPAEPPLLSKLDLPAAIVDAIAPQEGVVYVTGATGSGKSTLLAAIIRELAEAPDSHRKILTYEAPIEYVYDSLTTPTASVCQSEIPRHLNSFAAGVRNALRRKPHAILVGEARDNETISAVLEAALTGHPVYTTLHSSGVAETIRRLVGSFPAEERIGRTIDIIETLRLIIWQRLVPSVDGKRVALREFLVFKEEIRDLLLDSDPEQITAAVRRLVAEHGQPMQVDVEAKFKGGLISERLYKILSASQE |
| 预测分子量 | 45.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为假设性示例文献，供参考格式(实际文献需通过学术数据库查询)：
1. **"Structural characterization of the Legionella DotB ATPase in the type IV secretion system"**
- Authors: Smith J, et al.
- 摘要：通过X射线晶体学解析了DotB重组蛋白的三维结构，揭示其ATP酶活性位点及在分泌系统组装中的作用。
2. **"Functional analysis of recombinant DotB in bacterial pathogenesis"**
- Authors: Lee H, et al.
- 摘要：利用重组DotB蛋白进行体外实验，证明其通过水解ATP驱动效应蛋白转运，促进宿主细胞内细菌存活。
3. **"DotB interaction with host cell components during Legionella infection"**
- Authors: García R, et al.
- 摘要：通过免疫共沉淀技术发现重组DotB蛋白与宿主细胞内膜蛋白相互作用，揭示其在调控囊泡运输中的功能。
4. **"Expression and purification optimization of recombinant DotB for drug screening"**
- Authors: Chen L, et al.
- 摘要：优化了DotB重组蛋白的大肠杆菌表达体系，获得高纯度蛋白用于基于结构的抗感染药物开发。
**注意事项**：
- 以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索关键词(如"DotB protein"、"Legionella secretion system")。
- DotB研究多集中在细菌分泌系统机制领域，建议结合具体研究方向筛选文献。
**Background of DotB Recombinant Protein**
DotB is a key component of the Type IV secretion system (T4SS), a multi-protein complex critical for bacterial pathogenesis and horizontal gene transfer. It is notably associated with pathogens like *Legionella pneumophila*, which employs the T4SS to inject effector proteins into host cells, hijacking cellular processes to establish infection. The *dotB* gene encodes an ATPase that provides energy for the secretion machinery, facilitating the transport of virulence factors across bacterial and host membranes.
Recombinant DotB protein is produced through genetic engineering, often using *E. coli* expression systems. Its purification enables functional and structural studies, such as elucidating ATP hydrolysis mechanisms or interactions with other T4SS components. Structurally, DotB contains conserved AAA+ ATPase domains, which are essential for its role in assembling the secretion apparatus and mediating substrate translocation.
Research on DotB recombinant protein has advanced understanding of bacterial infection strategies. Inhibiting DotB activity has been explored as a therapeutic target to disrupt pathogenicity, particularly for diseases like Legionnaires’ disease. Additionally, studies on DotB contribute to broader insights into T4SS evolution and its role in antibiotic resistance gene dissemination.
Overall, DotB recombinant protein serves as a vital tool for dissecting microbial virulence mechanisms and developing anti-infective strategies, bridging molecular microbiology and translational medicine.
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