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Recombinant Human E1A91 protein

  • 中文名: (E1A91)重组蛋白
  • 别    名: E1A91;
货号: PA2000-4713
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纯度>90%SDS-PAGE.
种属Human
靶点E1A91
Uniprot NoA0A5D2ZVD2 
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-562aa
氨基酸序列MSTGMSKLGDKPEAAPNDAADYEDEEEDLSWSSDSEIVEALDYLDSKVDDEFIDSSFTLASRRPNAHGGVHSRPNSSALQPLSNRNQKFSNHIRASPLEEWEGRINVGMSNSVTTAIRESVREMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYHATKSDGQELAIKVYKTSVLVFKDRDRYVQGDYRFRYGYCKHNPRKMVKTWAEKEMRNLMRLKAAGIRCPTPLLLRLHVLVMEFIGKAGWAAPRLKVAALSLDKLRECYVEMIVAMQTLYQKCKLVHGDLSEYNILFYEGHLYIIDVSQAVDLDHPHALDFLREDCIHVSDFFKKHGVAVMTIRELFDFIVDPTITDGSVDSYLEKVQQKILARGDMSVEDEIADSVFVQTYIPKTLDHVKNVEEDVIRITSGKDTGDLYYKTITGLKEALPKDQSSPMEKQQGDTNTRSVEGSMGNPSGHSNSQESESESESPSGSETDGDEENLSGSEGKGPLSDSSAQVDKKAARKENKKKVKEEKREARKTKVPKAVKKRKKKLAKAHKTR
预测分子量63,1 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于E1A91重组蛋白的虚构参考文献示例,涵盖不同研究方向:

1. **《E1A91重组蛋白通过调控细胞周期抑制肿瘤生长的机制研究》**

*作者:Zhang, L. et al.*

摘要:本研究探讨了E1A91重组蛋白在乳腺癌模型中的抗癌作用。实验表明,该蛋白通过结合pRb蛋白并下调Cyclin D1表达,诱导G1期阻滞,显著抑制肿瘤细胞增殖。体内实验证实其能减少小鼠肿瘤体积,提示其作为潜在抗癌药物的价值。

2. **《E1A91重组蛋白的晶体结构解析及其功能域分析》**

*作者:Smith, J.R. & Kumar, S.*

摘要:通过X射线晶体学首次解析了E1A91重组蛋白的三维结构(分辨率2.1Å),发现其CR2结构域存在独特的α螺旋构象。该结构解释了其与宿主转录因子相互作用的分子基础,为靶向药物设计提供依据。

3. **《E1A91重组蛋白增强腺病毒载体基因治疗效率的研究》**

*作者:Lee, H. et al.*

摘要:将E1A91重组蛋白与腺病毒载体联用,发现其通过抑制组蛋白去乙酰化酶(HDAC)提高治疗基因表达效率。在肝癌模型中,联合疗法使肿瘤凋亡率提升40%,为基因治疗策略优化提供新思路。

4. **《E1A91与p53蛋白互作在细胞凋亡中的双重调控作用》**

*作者:Ivanova, T. & Petrov, V.*

摘要:研究揭示了E1A91重组蛋白通过N端结构域结合p53.在DNA损伤条件下协同激活促凋亡基因BAX,但在正常细胞中通过泛素化途径降解p53.这种双重调控机制为其选择性抗癌应用奠定理论基础。

注:以上内容为模拟示例,实际文献需通过学术数据库检索。

背景信息

**Background of E1A91 Recombinant Protein**

The E1A91 recombinant protein is derived from the adenovirus early region 1A (E1A), a multifunctional viral oncoprotein critical for adenovirus replication and cellular transformation. E1A is well-studied for its ability to modulate host cell processes by interacting with key regulatory proteins, including retinoblastoma (pRb) family members and transcriptional coactivators like p300/CBP. These interactions disrupt cell cycle checkpoints, promote S-phase entry, and alter gene expression, facilitating viral replication in infected host cells.

The E1A91 variant represents a truncated or modified form of the native E1A protein, often engineered for specific experimental applications. Recombinant E1A91 is typically produced using bacterial or mammalian expression systems, enabling high-purity yields for research use. Its design may include affinity tags (e.g., His or GST tags) to simplify purification and detection.

In research, E1A91 serves as a tool to study mechanisms of viral oncogenesis, cell cycle regulation, and protein-protein interactions. It has been pivotal in elucidating how viral proteins hijack cellular pathways, particularly in cancer biology, where E1A's dual role as an oncoprotein and tumor suppressor (depending on context) provides insights into cellular transformation and apoptosis. Additionally, E1A91 is used to explore therapeutic strategies, such as targeting E1A-interacting pathways in oncology or improving adenovirus-based gene therapy vectors.

The recombinant protein's utility lies in its ability to mimic or inhibit native E1A functions, offering a controlled system to dissect complex cellular mechanisms. Its applications span molecular biology, virology, and cancer research, underscoring its importance as a model for understanding host-pathogen interactions and cellular regulation.

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