| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Toxoplasma gondii surface antigen 2B |
| Entrez GeneID | KC607825.1 |
| clone | 1D12B8 |
| WB Predicted band size | 13.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of Toxoplasma gondii surface antigen 2B SAG2 (AA: 1-130) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于SAG2抗体的3篇示例参考文献(内容为虚构示例，实际文献需查证)：
1. **文献名称**："Recombinant SAG2 antigen for serodiagnosis of Toxoplasma gondii infection"
**作者**：Kong, J.T. et al.
**摘要**：研究利用重组表达的SAG2蛋白开发ELISA检测方法，验证其在弓形虫感染患者血清中的敏感性和特异性，证明SAG2抗体在早期诊断中的潜力。
2. **文献名称**："Comparative evaluation of SAG1 and SAG2 antibodies in Toxoplasma gondii detection"
**作者**：Burg, L. et al.
**摘要**：比较SAG1与SAG2抗体在免疫印迹和间接荧光抗体试验中的表现，发现SAG2抗体对慢性感染阶段的检测更具特异性。
3. **文献名称**："Monoclonal antibody production against SAG2 epitopes for rapid diagnostic kits"
**作者**：Chen, Y. & Wang, H.
**摘要**：开发针对SAG2表位的单克隆抗体，用于胶体金免疫层析试纸条，实现弓形虫感染的现场快速筛查。
**备注**：以上为模拟内容，实际文献需通过PubMed、Google Scholar等平台以“SAG2 antibody Toxoplasma”为关键词检索。
**Background of SAG2 Antibody**
SAG2 (Surface Antigen 2) is a key protein associated with *Toxoplasma gondii*, an obligate intracellular parasite responsible for toxoplasmosis. As a member of the SAG1-related gene family (SRS), SAG2 is predominantly expressed on the surface of the parasite’s tachyzoite stage, playing a critical role in host cell adhesion, immune evasion, and invasion. Its immunodominant nature makes it a major target for diagnostic and research applications.
Antibodies against SAG2 are widely used in serological assays to detect *T. gondii* infection. IgG and IgM antibodies specific to SAG2 help distinguish acute from chronic infections, aiding clinical management, particularly in pregnant women and immunocompromised individuals. Recombinant SAG2 or synthetic peptides are often employed in ELISA or immunoblotting to ensure high specificity and sensitivity.
Research on SAG2 antibodies also contributes to vaccine development, as SAG2 is a candidate antigen for inducing protective immunity. Monoclonal antibodies against SAG2 are valuable tools for studying parasite biology, including host-pathogen interactions and stage-specific antigen expression. However, genetic diversity in SAG2 across *T. gondii* strains may influence antibody binding efficiency, necessitating careful validation in diagnostic and research settings.
Overall, SAG2 antibodies are indispensable in understanding toxoplasmosis pathogenesis, improving diagnostics, and advancing therapeutic strategies against this globally prevalent infection.
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