| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50 - 1/200 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | NFM; NEF3; NF-M |
| Entrez GeneID | 4741 |
| clone | 1B6B12 |
| WB Predicted band size | 102.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NEFM (AA: 779-916) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于NEFM(神经丝中分子量亚基)抗体的代表性文献，简要概括研究内容：
1. **"Neurofilament markers in CSF and serum as diagnostic tools for ALS"**
- **作者**: G. Comi et al. (2021)
- **摘要**：研究探讨了NEFM抗体检测脑脊液和血清中神经丝蛋白的临床应用，发现NEFM与磷酸化神经丝蛋白(pNfH)联合检测可提高肌萎缩侧索硬化症(ALS)的早期诊断准确率。
2. **"Axonal neurofilament phosphorylation: a biomarker for chemotherapy-induced neurodegeneration"**
- **作者**: L.E. Goldstein et al. (2019)
- **摘要**：通过NEFM抗体和磷酸化特异性抗体分析发现，化疗药物会导致神经丝蛋白异常磷酸化，提示其可作为化疗相关神经毒性的生物标志物。
3. **"Validation of commercial neurofilament antibodies for post-mortem diagnostics"**
- **作者**: K.J. Smith et al. (2017)
- **摘要**：系统评估了市售NEFM抗体的特异性及灵敏度，证实其在尸检组织中可有效标记轴突损伤，适用于阿尔茨海默病等神经退行性病变的病理分析。
注：以上为文献内容概括，实际引用时建议通过PubMed或Google Scholar核对完整信息。NEFM抗体研究多集中于神经退行性疾病诊断、轴突损伤评估及抗体试剂验证等领域。
Neurofilament medium (NEFM) antibodies are essential tools in neuroscience research, targeting the medium subunit of neurofilaments (NFs), a key component of the neuronal cytoskeleton. Neurofilaments, classified into light (NEFL), medium (NEFM), and heavy (NEFH) chains based on molecular weight, form heteropolymers critical for maintaining axonal structure, caliber, and mechanical stability. NEFM, with a molecular weight of ~160 kDa, contributes to filament assembly and interacts with other cytoskeletal elements to regulate intracellular transport and neuronal integrity.
NEFM antibodies are widely used to study neuronal development, degeneration, and pathology. They help visualize axons in immunohistochemistry, detect neurofilament alterations in diseases like amyotrophic lateral sclerosis (ALS), Alzheimer’s disease, and traumatic brain injury, and serve as biomarkers in cerebrospinal fluid or blood to assess axonal damage. Dysregulated NEFM expression or abnormal phosphorylation is linked to disrupted axonal transport and neurodegeneration.
These antibodies also aid in distinguishing neuronal cells from glia in mixed cultures and exploring mechanisms underlying neurofilament-related disorders. Their specificity and reliability make them indispensable for both basic research and clinical diagnostics in neurology.
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