| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | preT |
| Uniprot No | P76440 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-412aa |
| 氨基酸序列 | MPQQNYLDELTPAFTSLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQRFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPGGWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVGLSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELTMAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAEAIHHYLEGACSC |
| 预测分子量 | 51.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于pre-T重组蛋白的3篇代表性文献示例(注：文献信息为假设性概括，仅作参考)：
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1. **文献名称**: *The pre-T cell receptor signaling in early T cell development*
**作者**: von Boehmer H., et al.
**摘要**: 探讨pre-TCR重组蛋白在T细胞发育早期阶段的关键作用，包括通过β选择促进细胞增殖与存活，并阻止未成熟T细胞的凋亡。研究揭示了pre-TCR信号对T细胞谱系定向的调控机制。
2. **文献名称**: *Recombinant pre-TCRα protein enhances thymocyte differentiation in vitro*
**作者**: Saint-Ruf C., et al.
**摘要**: 通过体外表达重组pre-TCRα蛋白，验证其与pTα链的结合能力及其对未成熟胸腺细胞分化的促进作用，为研究T细胞发育的分子机制提供实验工具。
3. **文献名称**: *Structural insights into pre-TCR assembly using recombinant protein models*
**作者**: Wang X., et al.
**摘要**: 利用重组pre-TCR蛋白进行X射线晶体学研究，解析其三维结构，阐明pre-TCR与配体相互作用的分子基础，为靶向T细胞发育异常的疾病治疗提供结构依据。
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**说明**：以上文献主题覆盖pre-TCR的生物学功能、体外实验应用及结构研究，实际检索时可通过PubMed/Google Scholar以关键词“pre-TCR recombinant protein”或“pre-T cell receptor”筛选近年高引论文。
**Background of Pre-T Recombinant Proteins**
Recombinant proteins, engineered through genetic modification, are pivotal in biomedical research and therapeutic development. Pre-T recombinant proteins, specifically, are derived from components associated with the pre-T cell receptor (pre-TCR), a critical player in early T-cell development. The pre-TCR is expressed on immature thymocytes during the β-selection checkpoint, a stage ensuring the proper rearrangement of the T-cell receptor (TCR) β-chain. This receptor consists of a functionally rearranged TCRβ chain paired with a pre-Tα chain, facilitating survival, proliferation, and differentiation of precursor T-cells into double-positive (CD4+CD8+) thymocytes.
The production of pre-T recombinant proteins leverages recombinant DNA technology, where genes encoding pre-TCR subunits (e.g., TCRβ, pre-Tα) are cloned into expression vectors and expressed in host systems like *E. coli*, yeast, or mammalian cells. These proteins retain structural and functional features of native pre-TCR components, enabling studies on T-cell development, signaling pathways (e.g., LCK, ZAP70 kinases), and interactions with extracellular ligands.
Pre-T recombinant proteins have broad applications. In basic research, they help dissect mechanisms underlying β-selection failures linked to immunodeficiencies or leukemias. Therapeutically, they serve as tools for modulating T-cell maturation in adoptive cell therapies or regenerating functional T-cells in immunodeficiency disorders. Challenges include ensuring proper post-translational modifications (e.g., glycosylation) for biological activity, often requiring mammalian expression systems.
Overall, pre-T recombinant proteins bridge molecular biology and immunology, offering insights into T-cell biology and translational potential in immune-related therapies. Continued optimization of production methods and functional assays will expand their utility in both research and clinical settings.
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