| 纯度 | > 90 % Densitometry. |
| 种属 | Human |
| 靶点 | ALCAM |
| Uniprot No | P55991 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-516aa |
| 氨基酸序列 | WYTVNSAYGDTIIIPCRLDVPQNLMFGKWKYEKPDGSPVFIAFRSSTKKSVQYDDVPEYKDRLNLSENYTLSISNARISDEKRFVCMLVTEDNVFEAPTIVKVFKQPSKPEIVSKALFLETEQLKKLGDCISEDSYPDGNITWYRNGKVLHPLEGAVVIIFKKEMDPVTQLYTMTSTLEYKTTKADIQMPFTCSVTYYGPSGQKTIHSEQAVFDIYYPTEQVTIQVLPPKNAIKEGDNITLKCLGNGNPPPEEFLFYLPGQPEGIRSSNTYTLTDVRRNATGDYKCSLIDKKSMIASTAITVHYLDLSLNPSGEVTRQIGDALPVSCTISASRNATVVWMKDNIRLRSSPSFSSLHYQDAGNYVCETALQEVEGLKKRESLTLIVEGKPQIKMTKKTDPSGLSKTIICHVEGFPKPAIQWTITGSGSVINQTEESPYINGRYYSKIIISPEENVTLTCTAENQLERTVNSLNVSAISIPEHDEADEISD |
| 预测分子量 | 70.8kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与ALCAM重组蛋白相关的文献摘要概括(文献标题及内容均基于公开研究虚构整合，仅供示例参考)：
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1. **标题**: *Recombinant ALCAM extracellular domain modulates T-cell activation and suppresses autoimmune encephalomyelitis*
**作者**: Li, X. et al.
**摘要**: 研究团队通过哺乳动物细胞表达系统制备了重组ALCAM胞外域蛋白，发现其可抑制T细胞受体介导的增殖反应，并在实验性自身免疫性脑脊髓炎(EAE)模型中显著减轻炎症反应，提示其潜在免疫调节应用价值。
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2. **标题**: *Structural characterization of human ALCAM immunoglobulin domains and its role in cancer cell adhesion*
**作者**: Gupta, R. & Park, S.
**摘要**: 利用重组ALCAM蛋白进行晶体结构解析，揭示了其Ig-like结构域的关键结合位点。体外实验证实，重组蛋白可竞争性阻断肿瘤细胞与内皮细胞的黏附，为抑制转移提供了分子机制依据。
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3. **标题**: *High-yield production of soluble ALCAM in E. coli for axonal regeneration studies*
**作者**: Müller, J. et al.
**摘要**: 通过优化大肠杆菌表达系统，实现了重组ALCAM的高效可溶性表达。该蛋白在神经元培养中显著促进轴突延伸，表明其在神经损伤修复中的潜在治疗作用。
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如需真实文献，建议通过PubMed(https://pubmed.ncbi.nlm.nih.gov)检索关键词 "recombinant ALCAM protein" 或结合具体研究领域筛选。
**Background of ALCAM Recombinant Protein**
ALCAM (Activated Leukocyte Cell Adhesion Molecule), also known as CD166. is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. It plays critical roles in cell-cell adhesion, immune response, and cellular migration by mediating homophilic (ALCAM-ALCAM) and heterophilic (e.g., ALCAM-CD6) interactions. ALCAM is expressed in various tissues, including the nervous, immune, and vascular systems, and is involved in processes such as neural development, T-cell activation, and leukocyte trafficking. Dysregulation of ALCAM has been linked to cancer progression, inflammation, and neurodegenerative diseases, highlighting its therapeutic and diagnostic potential.
Recombinant ALCAM protein is engineered through genetic modification, typically using mammalian or insect cell expression systems to ensure proper post-translational modifications (e.g., glycosylation). The purified protein often includes the extracellular domain, retaining functional epitopes for binding studies. It serves as a vital tool for investigating ALCAM-mediated signaling pathways, ligand-receptor interactions, and its role in disease mechanisms. Researchers utilize it in ELISA, flow cytometry, cell adhesion assays, and as an immunogen for antibody production.
The development of ALCAM recombinant protein has advanced research into its dual role in promoting cell adhesion (in homeostasis) versus enhancing metastatic potential in cancers. Studies also explore its utility as a biomarker for diseases like melanoma or Alzheimer’s, and its targeting in immunotherapies. Overall, ALCAM recombinant protein is a key reagent for deciphering the molecule’s biological functions and therapeutic applications.
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