Recombinant Mouse H2-K1 protein

中文名： I型表皮角蛋白Ha2(H2-K1)重组蛋白
别名： H2-K1；HHA2；HKA2；KRTHA2；Keratin, type I cuticular Ha2

货号: PA2000-4689

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Mouse
靶点	H2-K1
Uniprot No	P01901
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	22-305aa
氨基酸序列	GPHSLRYFVTAVSRPGLGEPRYMEVGYVDDTEFVRFDSDAENPRYEPRARWMEQEGPEYWERETQKAKGNEQSFRVDLRTLLGYYNQSKGGSHTIQVISGCEVGSDGRLLRGYQQYAYDGCDYIALNEDLKTWTAADMAALITKHKWEQAGEAERLRAYLEGTCVEWLRRYLKNGNATLLRTDSPKAHVTHHSRPEDKVTLRCWALGFYPADITLTWQLNGEELIQDMELVETRPAGDGTFQKWASVVVPLGKEQYYTCHVYHQGLPEPLTLRWEPPPSTVSNM
预测分子量	38.6 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于H2-K1重组蛋白的假设性参考文献示例(仅供学术写作参考，具体文献需通过数据库检索确认)：

1. **标题**: "Expression and purification of recombinant H2-K1 for structural studies"

**作者**: Smith A, et al.

**摘要**: 本研究描述了一种利用大肠杆菌表达系统高效表达小鼠MHC I类分子H2-K1重组蛋白的方法，并通过亲和层析和尺寸排阻色谱进行纯化，获得高纯度蛋白用于后续晶体学分析。

2. **标题**: "H2-K1/peptide complex structure reveals mechanisms of antigen presentation"

**作者**: Tanaka K, et al.

**摘要**: 通过X射线晶体学解析H2-K1重组蛋白与病毒抗原肽的复合物结构，揭示了该MHC分子结合抗原的关键氨基酸残基及构象变化，为疫苗设计提供结构基础。

3. **标题**: "Recombinant H2-K1 tetramers for tracking virus-specific CD8+ T cells"

**作者**: Chen L, et al.

**摘要**: 开发了基于H2-K1重组蛋白的四聚体技术，成功检测小鼠模型中流感病毒特异性T细胞应答，证明其在免疫监测中的应用潜力。

4. **标题**: "H2-K1-mediated cross-presentation in dendritic cells enhances antitumor immunity"

**作者**: Müller R, et al.

**摘要**: 利用重组H2-K1蛋白研究树突状细胞的抗原交叉呈递机制，发现其通过促进肿瘤抗原递递显著增强CD8+ T细胞抗肿瘤活性。

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**提示**：实际文献需通过PubMed/Google Scholar检索关键词(如"H2-K1 recombinant protein"+"expression"/"structure"/"immune")。建议优先选择《Nature Immunology》《Journal of Immunology》等期刊近10年高被引论文。

背景信息

**Background of H2-K1 Recombinant Protein**

H2-K1 is a classical major histocompatibility complex (MHC) class I molecule in mice, encoded by the *H2-K* gene located within the H2 complex on chromosome 17. As a key component of the adaptive immune system, MHC class I molecules like H2-K1 present peptide antigens derived from intracellular pathogens or abnormal cellular proteins (e.g., viral or tumor-associated antigens) to CD8+ cytotoxic T lymphocytes (CTLs), triggering immune responses. The H2-K1 protein consists of a polymorphic heavy chain (α chain) non-covalently associated with β2-microglobulin (β2m), forming a peptide-binding groove that accommodates antigenic peptides typically 8–10 amino acids long.

Recombinant H2-K1 proteins are engineered in vitro to study its structure, peptide-binding specificity, and interactions with T-cell receptors (TCRs) or immune modulators. These proteins are often produced in mammalian, insect, or bacterial expression systems, sometimes fused with tags (e.g., His-tag) for purification. Soluble forms of H2-K1. such as refolded monomers or tetramers, are widely used in immunological assays, including tetramer staining to detect antigen-specific CTLs or in vitro peptide-binding studies.

H2-K1 is highly polymorphic, with allelic variants influencing peptide presentation and immune recognition. Research on recombinant H2-K1 has advanced understanding of MHC-I-restricted immunity, autoimmune mechanisms, and vaccine design. Additionally, structural studies using recombinant H2-K1 have elucidated mechanisms of peptide-MHC interactions and TCR engagement, providing insights into cross-reactivity and immune evasion. Its role in mouse models makes it a critical tool for preclinical studies in infectious diseases, cancer immunotherapy, and transplantation biology.

Overall, H2-K1 recombinant proteins serve as indispensable reagents for dissecting MHC-I biology and developing targeted immunotherapies.