| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RD3 |
| Uniprot No | Q7Z3Z2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-195aa |
| 氨基酸序列 | MSLISWLRWNEAPSRLSTRSPAEMVLETLMMELTGQMREAERQQRERSNAVRKVCTGVDYSWLASTPRSTYDLSPIERLQLEDVCVKIHPSYCGPAILRFRQLLAEQEPEVQEVSQLFRSVLQEVLERMKQEEEAHKLTRQWSLRPRGSLATFKTRARISPFASDIRTISEDVERDTPPPLRSWSMPEFRAPKAD |
| 预测分子量 | 30.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RD3重组蛋白的3篇参考文献的简要信息：
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1. **文献名称**: "Expression and Purification of Recombinant RD3 Protein in Escherichia coli"
**作者**: Smith J, et al.
**摘要**: 本研究成功构建了RD3基因的原核表达载体，利用大肠杆菌系统实现了高效表达。通过亲和层析纯化获得高纯度重组RD3蛋白，并验证其抗原性，为后续抗体开发及功能研究奠定基础。
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2. **文献名称**: "Structural Characterization of RD3 and Its Role in Cellular Trafficking"
**作者**: Lee S, Kim D.
**摘要**: 通过X射线晶体学解析了重组RD3蛋白的三维结构，揭示其与视紫红质转运相关的关键结构域。实验表明RD3通过调控膜蛋白运输参与光感受器细胞功能，为视网膜病变机制提供新见解。
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3. **文献名称**: "RD3 Recombinant Protein Attenuates Tumor Growth via Modulating Wnt/β-catenin Signaling"
**作者**: Chen L, et al.
**摘要**: 在结肠癌模型中验证重组RD3蛋白可通过抑制Wnt/β-catenin通路抑制肿瘤增殖。体外实验显示RD3与β-catenin直接互作，提示其作为潜在抗癌治疗靶点的可能性。
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注：以上文献信息为模拟示例，实际研究中建议通过PubMed或Web of Science以关键词“RD3 recombinant protein”检索最新文献。若需具体文章，可提供更详细的研究背景或应用方向以便精准推荐。
**Background of RD3 Recombinant Protein**
RD3 (Retinal Degeneration 3) is a critical protein predominantly expressed in retinal photoreceptor cells, playing a vital role in maintaining retinal health and function. It was first identified through studies linking mutations in the *RD3* gene to Leber congenital amaurosis (LCA), a severe inherited retinal disorder causing early-onset vision loss. The RD3 protein is encoded by the *RD3* gene, located on human chromosome 1q32.1. and is essential for the proper localization and function of guanylate cyclase (GC), an enzyme crucial for phototransduction—the process converting light into electrical signals in the retina.
Structurally, RD3 is a small, highly conserved protein with a molecular weight of approximately 23 kDa. It acts as a chaperone, facilitating the trafficking of GC from the endoplasmic reticulum to the outer segments of photoreceptors. Without functional RD3. GC accumulates in the endoplasmic reticulum, disrupting the visual cycle and leading to photoreceptor degeneration. This mechanism underscores its role in retinal disease pathogenesis.
Recombinant RD3 protein is produced using biotechnological methods, often employing bacterial (e.g., *E. coli*) or mammalian expression systems to ensure proper folding and post-translational modifications. Purification techniques like affinity chromatography yield high-purity RD3. enabling its use in research and therapeutic development.
Beyond its association with LCA, RD3 is studied for broader implications in retinal degenerative diseases, including retinitis pigmentosa. Researchers utilize recombinant RD3 to investigate disease mechanisms, develop gene therapies, and explore protein replacement strategies. Its potential as a biomarker or therapeutic agent highlights its significance in ophthalmology and molecular medicine.
In summary, RD3 recombinant protein serves as a vital tool for understanding retinal biology, disease pathways, and advancing treatments for inherited blindness.
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