| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Znf410 |
| Uniprot No | Q86VK4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-478aa |
| 氨基酸序列 | MLSDELESKPELLVQFVQNTSIPLGQGLVESEAKDITCLSLLPVTEASECSRLMLPDDTTNHSNSSKEVPSSAVLRSLRVNVGPDGEETRAQTVQKSPEFLSTSESSSLLQDLQPSDSTSFILLNLTRAGLGSSAEHLVFVQDEAEDSGNDFLSSESTDSSIPWFLRVQELAHDSLIAATRAQLAKNAKTSSNGENVHLGSGDGQSKDSGPLPQVEKKLKCTVEGCDRTFVWPAHFKYHLKTHRNDRSFICPAEGCGKSFYVLQRLKVHMRTHNGEKPFMCHESGCGKQFTTAGNLKNHRRIHTGEKPFLCEAQGCGRSFAEYSSLRKHLVVHSGEKPHQCQVCGKTFSQSGSRNVHMRKHHLQLGAAGSQEQEQTAEPLMGSSLLEEASVPSKNLVSMNSQPSLGGESLNLPNTNSILGVDDEVLAEGSPRSLSSVPDVTHHLVTMQSGRQSYEVSVLTAVNPQELLNQGDLTERRT |
| 预测分子量 | 55.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Znf410重组蛋白的假设参考文献示例(注：内容为虚构，仅供参考格式)：
1. **文献名称**: "Structural and Functional Characterization of Recombinant Znf410 as a Transcriptional Regulator in Hematopoiesis"
**作者**: L. Chen et al.
**摘要**: 本研究利用大肠杆菌系统表达了重组Znf410蛋白，通过X射线晶体学解析其三维结构，发现其锌指结构域特异结合DNA特定序列。功能实验表明Znf410通过调控造血相关基因(如GATA1)影响红细胞分化。
2. **文献名称**: "Znf410 Knockdown Disrupts Epigenetic Silencing via Interaction with the NuRD Complex"
**作者**: M. Rodriguez et al.
**摘要**: 文章报道重组Znf410蛋白与NuRD复合体协同作用，通过染色质重塑调控基因沉默。实验显示Znf410缺失导致组蛋白修饰异常，影响胚胎干细胞多能性维持。
3. **文献名称**: "Development of a High-Yield Expression System for Recombinant Znf410 and Its Application in Sickle Cell Disease Models"
**作者**: T. Watanabe & S. Patel
**摘要**: 研究者优化了昆虫细胞表达系统，高效纯化重组Znf410.并在镰刀细胞贫血模型中验证其通过激活γ-珠蛋白表达缓解病理表型的治疗潜力。
(注：以上文献为示例，实际文献需通过学术数据库检索。)
**Background of Znf410 Recombinant Protein**
Zinc finger protein 410 (ZNF410) is a transcription factor characterized by its multiple C2H2-type zinc finger domains, which enable sequence-specific DNA binding. It is evolutionarily conserved and primarily implicated in transcriptional regulation, particularly in hematopoiesis and cellular differentiation. ZNF410 gained attention due to its unique role in regulating the expression of *CHD4*, a core subunit of the NuRD (Nucleosome Remodeling and Deacetylase) complex, which is critical for chromatin remodeling and gene silencing. Studies suggest that ZNF410 binds directly to the promoter region of *CHD4*, maintaining its expression levels and thereby influencing NuRD-mediated epigenetic regulation.
Recombinant ZNF410 protein is engineered for in vitro and in vivo studies to dissect its molecular interactions, DNA-binding specificity, and functional mechanisms. Produced using expression systems like *E. coli* or mammalian cells, the recombinant protein often includes affinity tags (e.g., His-tag) for purification and detection. Its applications span structural studies (e.g., crystallography), electrophoretic mobility shift assays (EMSAs) to map DNA-binding sites, and cellular assays to explore its role in pathways linked to erythropoiesis or cancer.
Notably, dysregulation of ZNF410 has been associated with hematologic disorders, including myelodysplastic syndromes (MDS), where altered CHD4 levels disrupt normal blood cell development. Recombinant ZNF410 tools aid in identifying therapeutic targets or biomarkers for such conditions. Ongoing research also investigates its potential interplay with other zinc finger proteins and transcription factors, highlighting its broader regulatory network in cellular homeostasis.
In summary, ZNF410 recombinant protein serves as a key reagent for unraveling the transcription factor’s role in epigenetics, development, and disease, offering insights for both basic biology and translational medicine.
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