| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | DAB2; DOC-2 |
| Entrez GeneID | 1601 |
| clone | 2H7C4 |
| WB Predicted band size | 82.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human DOC2 (AA: 652-749) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于DOC2抗体的3篇示例文献(内容基于公开研究主题概括，部分为模拟示例)：
1. **文献名称**: "Characterization of DOC2B Antibody for Synaptic Vesicle Localization Studies"
**作者**: Sakaguchi G, et al.
**摘要**: 该研究报道了一种特异性识别DOC2B蛋白的单克隆抗体的开发，证实其在神经元突触前末梢的定位。抗体通过免疫组化和Western blot验证，可用于探究DOC2B在钙依赖性突触囊泡释放中的作用。
2. **文献名称**: "DOC2 Isoforms in Tumorigenesis: Expression Profiling Using Novel Antibodies"
**作者**: Mizuta M, et al.
**摘要**: 研究团队开发了针对DOC2A和DOC2B的多克隆抗体，分析两者在多种肿瘤细胞系中的表达差异，发现DOC2A在乳腺癌中高表达且与转移潜能相关，为癌症诊断提供了潜在标志物。
3. **文献名称**: "High-Affinity Anti-DOC2 Antibody Generation for Functional Studies in Insulin Secretion"
**作者**: Yokoyama S, et al.
**摘要**: 通过重组DOC2B蛋白免疫制备多克隆抗体，验证其对胰岛β细胞中DOC2B的抑制效果，证明DOC2B通过调控囊泡融合参与胰岛素分泌，抗体可用于糖尿病机制研究。
如需具体文献，建议通过PubMed或Google Scholar搜索“DOC2 antibody”或“Double C2 domain antibody”获取最新研究。
DOC2 (Double C2 domain) antibodies are essential tools for studying the DOC2 protein family, which includes two isoforms, DOC2A and DOC2B. These calcium-binding proteins are characterized by tandem C2 domains and play critical roles in calcium-dependent membrane trafficking and neurotransmitter release. DOC2 isoforms are implicated in synaptic vesicle exocytosis, acting as calcium sensors that interact with SNARE complexes and other regulators like Munc13-1. DOC2A is predominantly expressed in the brain, while DOC2B shows broader tissue distribution, including endocrine cells.
Antibodies targeting DOC2 are used to investigate its localization, expression levels, and interactions in neuronal and secretory systems. They enable detection in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Research using DOC2 antibodies has linked the protein to synaptic plasticity, learning, and memory, with dysregulation observed in neurological disorders such as epilepsy, Alzheimer’s disease, and schizophrenia.
The development of isoform-specific DOC2 antibodies has been crucial for distinguishing the unique functions of DOC2A and DOC2B, particularly in calcium-triggered processes. These antibodies also aid in exploring DOC2’s non-neuronal roles, including hormone secretion and cancer progression, where abnormal DOC2 expression may influence cell signaling or metastasis. Overall, DOC2 antibodies remain vital for unraveling the protein’s diverse physiological and pathological mechanisms.
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