| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HPCA |
| Uniprot No | P84074 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-193aa |
| 氨基酸序列 | GKQNSKLRPEMLQDLRENTEFSELELQEWYKGFLKDCPTGILNVDEFKKIYANFFPYGDASKFAEHVFRTFDTNSDGTIDFREFIIALSVTSRGRLEQKLMWAFSMYDLDGNGYISREEMLEIVQAIYKMVSSVMKMPEDESTPEKRTEKIFRQMDTNNDGKLSLEEFIRGAKSDPSIVRLLQCDPSSASQF |
| 预测分子量 | 29.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HPCA(假设为Hippocalcin)重组蛋白研究的示例性参考文献摘要，仅供参考。建议通过学术数据库(如PubMed、Web of Science)检索具体文献：
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1. **文献名称**：*"High-yield expression and purification of recombinant human hippocalcin (HPCA) in Escherichia coli for structural studies"*
**作者**：Zhang, Y., et al.
**摘要**：本研究报道了一种高效的大肠杆菌表达系统，用于重组HPCA的制备，并通过亲和层析和离子交换法纯化获得高纯度蛋白。进一步利用圆二色光谱证实其钙离子结合能力，为后续结构生物学研究奠定基础。
2. **文献名称**：*"Functional characterization of recombinant HPCA in modulating neuronal calcium homeostasis"*
**作者**：Chen, L., & Wang, H.
**摘要**：通过体外实验发现，重组HPCA可特异性结合Ca²⁺并调控神经元内钙稳态，抑制过量钙离子引发的凋亡通路，提示其在神经退行性疾病中的潜在保护机制。
3. **文献名称**：*"Development of a novel HPCA-based biosensor for real-time calcium imaging in live cells"*
**作者**：Kurokawa, T., et al.
**摘要**：研究团队将重组HPCA与荧光蛋白融合，开发出新型基因编码钙离子探针，成功应用于活细胞钙动态的高分辨率成像，灵敏度显著优于传统指标。
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**注**：以上为模拟示例，实际文献需根据具体研究方向检索。若HPCA指代其他蛋白(如HPV相关抗原)，建议补充背景信息以便精准推荐。
**Background of HPCA Recombinant Protein**
Hippocalcin (HPCA) is a neuronal calcium sensor protein belonging to the recoverin subfamily of EF-hand calcium-binding proteins. It is predominantly expressed in the brain, particularly in hippocampal neurons, and plays a critical role in regulating calcium-dependent signaling pathways. HPCA is involved in modulating neuronal excitability, synaptic plasticity, and neurotransmitter release, making it essential for learning, memory, and cognitive functions. Dysregulation of HPCA has been implicated in neurological disorders, including epilepsy and neurodegenerative diseases.
Recombinant HPCA protein is produced using genetic engineering techniques, where the HPCA gene is cloned into expression vectors and expressed in host systems like *E. coli* or mammalian cell lines. This allows large-scale production of the protein with high purity and consistency. The recombinant form retains the functional properties of native HPCA, including calcium-binding capacity and interactions with target molecules such as protein kinase C (PKC) and glutamate receptors.
Researchers utilize recombinant HPCA to study calcium signaling mechanisms, neuronal development, and disease pathology. It serves as a tool for in vitro assays, structural studies, and drug discovery, particularly in identifying compounds that modulate calcium-dependent processes. Additionally, recombinant HPCA aids in developing diagnostic biomarkers for neurological conditions. Its applications extend to understanding synaptic dysfunction and exploring therapeutic strategies for brain disorders.
In summary, recombinant HPCA provides a versatile platform for advancing neuroscience research and bridging gaps between molecular mechanisms and neurological health challenges.
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