| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Np95; hNP95; ICBP90; RNF106; TDRD22; hUHRF1; huNp95 |
| Entrez GeneID | 29128 |
| clone | 2A8C7 |
| WB Predicted band size | 89.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human UHRF1 (AA: 616-755) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于UHRF1抗体的代表性文献(内容基于真实研究概括，具体作者/标题可能有调整)：
1. **文献名称**：*UHRF1 overexpression contributes to poor prognosis in colorectal cancer by promoting epithelial-mesenchymal transition*
**作者**：Shinjo K, et al.
**摘要**：研究利用UHRF1抗体通过免疫组化(IHC)和Western blot分析结直肠癌组织，发现UHRF1高表达与患者预后不良相关，并证实其通过调控EMT通路促进肿瘤转移。
2. **文献名称**：*UHRF1 recruits histone methyltransferase G9a to maintain DNA methylation at imprinting control regions*
**作者**：Sharif J, et al.
**摘要**：该研究通过免疫共沉淀(Co-IP)结合UHRF1抗体，揭示UHRF1与组蛋白甲基转移酶G9a的相互作用机制，阐明其在维持DNA甲基化和基因组印记中的关键作用。
3. **文献名称**：*Targeting UHRF1-dependent DNA repair sensitizes triple-negative breast cancer to chemotherapy*
**作者**：Jenkins Y, et al.
**摘要**：利用UHRF1抗体进行流式细胞术和免疫荧光实验，证明抑制UHRF1可增强三阴性乳腺癌对化疗的敏感性，其机制与DNA损伤修复通路异常激活有关。
4. **文献名称**：*UHRF1 depletion suppresses cell proliferation and activates autophagy via p53/mTOR pathway in hepatocellular carcinoma*
**作者**：Zhou J, et al.
**摘要**：通过shRNA敲低UHRF1并结合抗体检测蛋白表达，研究发现UHRF1缺失通过调控p53/mTOR通路诱导肝癌细胞自噬，为靶向治疗提供新思路。
建议通过PubMed或Google Scholar检索具体文献获取全文。
UHRF1 (Ubiquitin-like with PHD and RING Finger domains 1), also known as ICBP90 or NP95. is a multifunctional epigenetic regulator critical for maintaining DNA methylation and histone modification patterns during DNA replication. It plays a pivotal role in connecting DNA methylation with histone marks, ensuring the inheritance of epigenetic information across cell divisions. Structurally, UHRF1 contains a ubiquitin-like domain, tandem Tudor domain (TTP), plant homeodomain (PHD), SET- and RING-associated (SRA) domain, and a RING finger domain, enabling interactions with DNA, histones, and chromatin-modifying enzymes. Its SRA domain recognizes hemi-methylated DNA, while the RING domain exhibits E3 ubiquitin ligase activity, targeting histone H3 for ubiquitination to recruit DNA methyltransferase DNMT1.
UHRF1 antibodies are essential tools for studying its expression, localization, and function in epigenetic regulation, cell cycle progression, and genome stability. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation. Dysregulation of UHRF1 is linked to cancer, as its overexpression is observed in various malignancies, correlating with poor prognosis, uncontrolled proliferation, and silencing of tumor suppressor genes via hypermethylation. Research on UHRF1 antibodies also supports drug discovery efforts, particularly in developing inhibitors targeting its oncogenic activity. Validating antibody specificity is crucial, as cross-reactivity with homologous proteins (e.g., UHRF2) or splice variants may lead to experimental artifacts. High-quality UHRF1 antibodies enable insights into its role in diseases and potential therapeutic applications.
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