| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SCGB2A1 |
| Uniprot No | O75556 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-95aa |
| 氨基酸序列 | DSGCKLLEDMVEKTINSDISIPEYKELLQEFIDSDAAAEAMGKFKQCFLNQSHRTLKNFGLMMHTVYDSIWCNMKSN |
| 预测分子量 | 14.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SCGB2A1重组蛋白的3篇参考文献示例(注：文献信息为模拟概括，仅供参考)：
1. **文献名称**: *"Recombinant SCGB2A1 Expression in Breast Cancer Cells Modulates Proliferation and Invasion*
**作者**: Ni, Y. et al.
**摘要**: 该研究通过构建SCGB2A1重组蛋白表达载体，在乳腺癌细胞系中过表达该蛋白，发现其显著抑制细胞增殖和迁移能力，提示SCGB2A1可能作为肿瘤抑制因子发挥作用。
2. **文献名称**: *"Production and Characterization of Recombinant Human SCGB2A1 in E. coli*
**作者**: Jackson, R. et al.
**摘要**: 本研究利用大肠杆菌表达系统成功制备高纯度SCGB2A1重组蛋白，并通过质谱和圆二色谱验证其正确折叠。该蛋白可用于后续抗体开发及功能研究。
3. **文献名称**: *"SCGB2A1 Recombinant Protein Attenuates Airway Inflammation via Suppressing NF-κB Signaling*
**作者**: Smith, L. et al.
**摘要**: 实验证明SCGB2A1重组蛋白能够抑制脂多糖诱导的气道上皮细胞炎症反应，其机制与阻断NF-κB通路的活化相关，为治疗呼吸系统疾病提供潜在策略。
4. **文献名称**: *"SCGB2A1 as a Potential Diagnostic Biomarker: Development of a Recombinant Protein-Based ELISA*
**作者**: Wang, H. et al.
**摘要**: 利用SCGB2A1重组蛋白建立新型ELISA检测方法，发现其在乳腺癌患者血清中的表达水平显著降低，验证了其作为诊断标志物的可行性。
**注意**：以上文献信息为示例性概括，实际引用时需以真实发表的文献为准。
Secretoglobin family 2A member 1 (SCGB2A1), also known as mammaglobin B, is a low-molecular-weight secretory protein belonging to the secretoglobin superfamily. This protein is structurally characterized by a conserved cysteine-rich domain forming disulfide bonds, which stabilizes its dimeric structure. Primarily expressed in epithelial tissues, SCGB2A1 is secreted into bodily fluids such as breast milk, saliva, and tears, suggesting roles in mucosal immunity and inflammation modulation. Its gene is located on chromosome 11q12.2. clustered with other secretoglobin family members.
SCGB2A1 has drawn attention for its tissue-specific expression patterns and potential clinical applications. While its exact physiological function remains unclear, studies associate it with anti-inflammatory properties, cell signaling regulation, and antimicrobial activity. Notably, SCGB2A1 is co-expressed with mammaglobin A (SCGB2A2) in mammary tissue, and both have been investigated as biomarkers for breast cancer detection due to their elevated expression in malignant breast tumors. The recombinant form of SCGB2A1 is typically produced using bacterial (e.g., E. coli) or mammalian expression systems, followed by purification via chromatography techniques. This engineered protein enables standardized research on its biochemical properties, receptor interactions, and therapeutic potential. Current investigations explore its utility in diagnostic assays, targeted cancer therapies, and as a component of multi-antigen vaccines. However, challenges persist in fully elucidating its in vivo mechanisms and clinical relevance across different pathological conditions.
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