| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | BXR; PAR; PRR; PXR; SAR; SXR; ONR1; PAR1; PAR2; PARq |
| Entrez GeneID | 8856 |
| clone | 1D12G1 |
| WB Predicted band size | 49.8kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NR1I2 (AA: 1-142) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下为模拟生成的关于NR1I2(PXR)抗体的参考文献示例，供参考：
---
1. **文献名称**：*Development and characterization of a monoclonal antibody specific for human NR1I2/PXR*
**作者**：Kliewer SA, et al.
**摘要**：本研究报道了一种针对人NR1I2/PXR蛋白的单克隆抗体的开发，通过免疫印迹(Western blot)和免疫组化验证其特异性，成功应用于检测肝脏和肠道组织中PXR的基础表达及药物诱导后的表达变化。
2. **文献名称**：*Comparative analysis of commercial anti-PXR antibodies for nuclear receptor studies*
**作者**：Moore JT, et al.
**摘要**：文章系统评估了多种市售NR1I2抗体的性能，发现部分抗体在染色质免疫沉淀(ChIP)和免疫荧光中存在交叉反应性，强调了抗体验证在核受体功能研究中的重要性，并推荐了适用于不同实验场景的可靠抗体。
3. **文献名称**：*PXR antibody-based profiling in drug-induced liver injury models*
**作者**：Xie W, et al.
**摘要**：利用特异性NR1I2抗体研究药物性肝损伤中PXR的调控机制，发现抗体可有效检测PXR与药物代谢酶(如CYP3A4)的共定位，为毒性代谢通路分析提供了工具支持。
4. **文献名称**：*A peptide-derived polyclonal antibody for detecting rodent PXR in pharmacokinetic studies*
**作者**：Staudinger JL, et al.
**摘要**：通过合成NR1I2羧基端多肽制备多克隆抗体，验证其在大鼠和小鼠肝组织中的特异性，成功应用于药代动力学研究中PXR蛋白水平的定量分析。
---
**注**：以上文献为模拟生成，实际引用时请通过学术数据库(如PubMed、Web of Science)检索真实存在的文献并核对信息。
The NR1I2 antibody is a crucial tool for studying the nuclear receptor subfamily 1. group I, member 2 (NR1I2), also known as the pregnane X receptor (PXR). PXR is a ligand-activated transcription factor that regulates the expression of genes involved in drug metabolism, detoxification, and transport, including cytochrome P450 enzymes (e.g., CYP3A4) and multidrug resistance proteins. It plays a central role in xenobiotic and bile acid homeostasis by sensing lipophilic compounds, such as pharmaceuticals, environmental toxins, and endogenous molecules, and orchestrating adaptive transcriptional responses.
NR1I2 antibodies are widely used in research to detect PXR expression, localization, and activity in tissues like the liver, intestine, and kidney. They enable techniques such as Western blotting, immunohistochemistry, and chromatin immunoprecipitation (ChIP) to investigate PXR's regulatory mechanisms, ligand interactions, and crosstalk with other nuclear receptors (e.g., CAR, FXR). These antibodies are essential for exploring PXR's role in drug-drug interactions, metabolic diseases, and cancer chemoresistance.
Given PXR's clinical relevance in modulating drug efficacy and toxicity, NR1I2 antibodies also support drug development efforts targeting PXR activation or inhibition to optimize therapeutic outcomes. Research using these antibodies continues to unravel PXR's complex roles in physiology and disease.
×
