| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAPN8 |
| Uniprot No | A6NHC0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-703aa |
| 氨基酸序列 | MAAQAAGVSRQRAATQGLGSNQNALKYLGQDFKTLRQQCLDSGVLFKDPEFPACPSALGYKDLGPGSPQTQGIIWKRPTELCPSPQFIVGGATRTDICQGGLGDCWLLAAIASLTLNEELLYRVVPRDQDFQENYAGIFHFQFWQYGEWVEVVIDDRLPTKNGQLLFLHSEQGNEFWSALLEKAYAKLNGCYEALAGGSTVEGFEDFTGGISEFYDLKKPPANLYQIIRKALCAGSLLGCSIDVSSAAEAEAITSQKLVKSHAYSVTGVEEVNFQGHPEKLIRLRNPWGEVEWSGAWSDDAPEWNHIDPRRKEELDKKVEDGEFWMSLSDFVRQFSRLEICNLSPDSLSSEEVHKWNLVLFNGHWTRGSTAGGCQNYPATYWTNPQFKIRLDEVDEDQEESIGEPCCTVLLGLMQKNRRWRKRIGQGMLSIGYAVYQVPKELESHTDAHLGRDFFLAYQPSARTSTYVNLREVSGRARLPPGEYLVVPSTFEPFKDGEFCLRVFSEKKAQALEIGDVVAGNPYEPHPSEVDQEDDQFRRLFEKLAGKDSEITANALKILLNEAFSKRTDIKFDGFNINTCREMISLLDSNGTGTLGAVEFKTLWLKIQKYLEIYWETDYNHSGTIDAHEMRTALRKAGFTLNSQVQQTIALRYACSKLGINFDSFVACMIRLETLFKLFSLLDEDKDGMVQLSLAEWLCCVLV |
| 预测分子量 | 79.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAPN8重组蛋白的3篇参考文献及其摘要内容：
1. **《Calpain-8 interacts with calpain-9 to promote proliferation in gastric cancer through activation of the Akt signaling pathway》**
- **作者**: Li Y, et al.
- **摘要**: 研究报道了CAPN8与CAPN9在胃癌细胞中的相互作用，利用重组蛋白技术证实两者协同激活Akt信号通路，促进肿瘤细胞增殖，提示其作为潜在治疗靶点。
2. **《Expression and characterization of recombinant human calpain-8: Unique substrate specificity compared to other calpain isoforms》**
- **作者**: Hata S, et al.
- **摘要**: 通过在大肠杆菌中表达重组人源CAPN8.发现其酶活性依赖钙离子且底物特异性不同于CAPN1/2.尤其偏好水解胃肠道相关蛋白，暗示其在消化系统中的独特功能。
3. **《Structural insights into calpain-8 activation and regulation by small molecules》**
- **作者**: Ono Y, et al.
- **摘要**: 利用重组CAPN8蛋白进行结构解析，揭示了其自抑制构象及激活机制，并筛选出特异性抑制剂，为开发靶向CAPN8的药物提供理论基础。
注：以上文献信息为模拟示例，实际引用需查询PubMed或专业数据库获取原文。
Calpain 8 (CAPN8) is a member of the calpain family of calcium-dependent cysteine proteases, which play critical roles in diverse cellular processes, including signal transduction, apoptosis, and cell proliferation. Encoded by the *CAPN8* gene, this enzyme is primarily expressed in the gastrointestinal tract, particularly in the stomach, where it is implicated in maintaining mucosal integrity and digestive functions. Unlike other calpain isoforms, CAPN8 forms a heterodimer with the regulatory subunit CAPN9. creating the gastric-specific calpain complex known as "G-calpain." This unique partnership is essential for its stability and enzymatic activity under physiological conditions.
Recombinant CAPN8 protein is engineered through molecular cloning techniques, often expressed in bacterial or mammalian systems to study its structure, regulation, and biological roles. Researchers utilize recombinant CAPN8 to investigate its protease activity, substrate specificity, and interactions with CAPN9. shedding light on its contribution to gastric homeostasis. Studies suggest that CAPN8 may participate in processing proteins involved in cell adhesion, mucus secretion, or repair mechanisms, though its precise substrates remain under exploration.
Dysregulation of CAPN8 has been linked to gastrointestinal pathologies, such as gastric cancer and inflammatory disorders, making it a potential therapeutic target. Recombinant CAPN8 also serves as a tool for screening inhibitors or modulators to develop targeted treatments. Its study bridges gaps in understanding tissue-specific calpain functions and their relevance to human health, emphasizing the need for further research into its molecular mechanisms and disease associations.
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