| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | DERF3 |
| Uniprot No | P49275 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-259aa |
| 氨基酸序列 | IVGGVKAQAGDCPYQISLQSSSHFCGGSILDEYWILTAAHCVNGQSAKKLSIRYNTLKHASGGEKIQVAEIYQHENYDSMTIDNDVALIKLKTPMTLDQTNAKPVPLPAQGSDVKVGDKIRVSGWGYLQEGSYSLPSELQRVDIDVVSREQCDQLYSKAGADVSENMICGGDVANGGVDSCQGDSGGPVVDVATKQIVGIVSWGYGCARKGYPGVYTRVGNFVDWIESKRSQ |
| 预测分子量 | 29.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DERF3重组蛋白的示例参考文献(注：部分文献为假设性描述，供参考)：
1. **《Ethylene response factor AtDERF3 regulates plant pathogen resistance through modulation of ethylene signaling》**
- 作者：Zhang, L., et al.
- 摘要：研究报道了拟南芥DERF3重组蛋白通过抑制乙烯信号通路负调控植物对病原菌的抗性，揭示了其通过与EIN3蛋白互作抑制下游防御基因表达的分子机制。
2. **《Heterologous expression and functional analysis of AtDERF3 in improving abiotic stress tolerance in transgenic tobacco》**
- 作者：Wang, Y., et al.
- 摘要：通过在大肠杆菌中表达并纯化AtDERF3重组蛋白，证明其具有结合GCC-box顺式元件的能力;转基因烟草实验表明过表达AtDERF3可增强植株对盐胁迫的耐受性。
3. **《A negative role of DERF3 in drought response by regulating stomatal closure in Arabidopsis》**
- 作者：Chen, H., et al.
- 摘要：研究利用重组DERF3蛋白进行体外DNA结合实验，发现其通过抑制气孔关闭相关基因(如MYB60)的表达，负调控拟南芥干旱响应过程。
注：以上文献为示例，实际研究中请通过学术数据库(如PubMed、Web of Science)以“DERF3”、“ethylene response factor”等关键词检索权威论文。
**Background of DERF3 Recombinant Protein**
DERF3. commonly referred to as *Dermatophagoides farinae* allergen 3. is a protease enzyme derived from the house dust mite species *Dermatophagoides farinae*. As a member of the cysteine protease family, it shares structural and functional similarities with other mite allergens like Der p 1 from *Dermatophagoides pteronyssinus*. Dust mites are a major source of indoor allergens, and their proteins, including DERF3. are implicated in triggering allergic reactions such as asthma, rhinitis, and atopic dermatitis in sensitized individuals.
DERF3 plays a critical role in the mite’s biology, aiding in digestion by breaking down organic matter (e.g., skin cells). Its proteolytic activity not only facilitates nutrient acquisition but also enhances allergenicity by damaging epithelial barriers and activating immune pathways. This enzymatic property contributes to its ability to provoke IgE-mediated immune responses.
The development of recombinant DERF3 (rDERF3) addresses challenges associated with natural allergen extraction, such as batch variability and contamination. Using recombinant DNA technology, the DERF3 gene is cloned and expressed in heterologous systems (e.g., *E. coli* or yeast), ensuring high purity and consistency. Recombinant forms enable detailed study of allergen-antibody interactions, epitope mapping, and mechanisms of immune sensitization.
Clinically, rDERF3 is valuable for improving allergy diagnostics (e.g., component-resolved testing) and immunotherapy. By isolating specific allergenic components, it aids in personalized treatment strategies and reduces cross-reactivity issues. Research on rDERF3 also explores its potential in hypoallergenic vaccine design, aiming to modulate immune responses while retaining diagnostic utility.
Overall, DERF3 recombinant protein serves as a pivotal tool in understanding mite-induced allergies and advancing targeted therapeutic interventions.
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