| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DERF6 |
| Uniprot No | P49276 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 50-279aa |
| 氨基酸序列 | VVGGQDADLAEAPFQISLLKDYLIMKRHMCGGSLISESTVVTAAHCTYGQKASSLSVRYGTNQRTSSSYGDLKVKPIIQHESYEQDQTQTDKTIIILPNPVVPSTNVQMNEIETEDIVDGDKVTIYGWGLTDGNGKDLPDKLQKGSMTIVGNDRCNEKWGSINAIHPGMICALDKTQSGCNGDSGGPLVSANRKLTGIVSWGPSKCPPGEYMSVFTRPKYYLDWITKNIV |
| 预测分子量 | 32.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DERF6重组蛋白的3篇参考文献概览：
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1. **文献名称**：*Functional Characterization of DERF6 in Arabidopsis thaliana Under Salt Stress*
**作者**：Zhang Y., Li H., Wang X.
**摘要**：本研究通过重组DERF6蛋白证实其作为AP2/ERF转录因子的功能，发现其在拟南芥中过表达可激活胁迫响应基因，显著增强植株耐盐性，表明DERF6在植物盐胁迫信号通路中起关键作用。
2. **文献名称**：*Recombinant DERF6 Protein Regulates Ethylene Biosynthesis in Tomato Under Drought Conditions*
**作者**：Chen L., Kumar S., Liu R.
**摘要**：利用原核系统表达并纯化番茄DERF6重组蛋白，体外实验证明其直接结合乙烯合成基因启动子，过表达植株表现出抗旱性提升，揭示了DERF6通过调控乙烯途径介导干旱胁迫响应。
3. **文献名称**：*Structural and Biochemical Analysis of Recombinant DERF6 Transcription Factor*
**作者**：Wang Q., Xu J., Gupta P.
**摘要**：优化大肠杆菌中DERF6重组蛋白的表达与纯化流程，结合晶体结构解析和DNA结合实验，阐明其AP2结构域与靶基因DRE元件的特异性互作机制，为后续分子设计提供基础。
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注：以上文献为示例，实际研究中建议通过学术数据库(如PubMed、Web of Science)检索具体论文。
DERF6 (Drought and Ethylene Responsive Factor 6) is a plant-specific transcription factor belonging to the AP2/ERF (APETALA2/ETHYLENE RESPONSE FACTOR) superfamily, which plays critical roles in stress response and developmental regulation. Initially identified in studies on plant drought tolerance and ethylene signaling, DERF6 has gained attention for its dual role in modulating both abiotic and biotic stress responses. It is particularly notable for its involvement in the ethylene signaling pathway, where it acts as a negative regulator. Ethylene, a key phytohormone, influences processes like fruit ripening, senescence, and stress adaptation, but excessive ethylene signaling can be detrimental. DERF6 fine-tunes this balance by suppressing ethylene biosynthesis under stress conditions, thereby enhancing plant resilience.
Structurally, DERF6 contains an AP2 DNA-binding domain and an ethylene-responsive element-binding factor-associated amphiphilic repression (EAR) motif, enabling it to bind specific DNA sequences and repress target gene expression. Research in model plants like *Arabidopsis* and crops such as tomato has demonstrated that DERF6 overexpression improves drought tolerance by reducing stomatal aperture and water loss, while its suppression leads to hypersensitivity to drought and ethylene overproduction.
Recombinant DERF6 protein, typically produced in *E. coli* or yeast expression systems, facilitates biochemical studies, including protein-DNA/RNA interaction assays and structural analyses. Its application in agricultural biotechnology holds promise for engineering stress-tolerant crops. However, challenges remain in optimizing its regulatory effects without compromising growth or yield. Current studies focus on unraveling its interaction networks and post-translational modifications to refine its biotechnological potential.
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