| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | iutA |
| Uniprot No | P14542 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-732aa |
| 氨基酸序列 | QQTDDETFVVSANRSNRTVAEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVLVDGVRLNSSRTDSRQLDSIDPFNMHHIEVIFGATSLYGGGSTGGLINIVTKKGQPETMMEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDNTQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTSTPFVSNGLNSDRIPGTDGHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQVTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGGLNNKKIYTTGRYPSYDITNLAAFLQSGYDINNLFTLNGGVRYQYTENKIDDFIGYAQQRQIGAGKATSADAFWRLSRLRHFLFNAGLLMHITEPQQAWLNFSQGLELPDPGKYYGRGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKSVVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDVKTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTLSFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF |
| 预测分子量 | 85.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于iutA重组蛋白的3篇参考文献示例(文献信息为虚构，仅供示例参考)：
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1. **标题**: *Cloning and Expression of the iutA Gene Encoding an Iron Receptor in Pathogenic E. coli*
**作者**: Smith J, et al.
**摘要**: 研究成功克隆iutA基因并在大肠杆菌中重组表达，纯化后的蛋白显示与铁载体结合活性，为后续研究细菌铁摄取机制奠定基础。
2. **标题**: *Immunogenicity of Recombinant iutA Protein as a Vaccine Candidate Against Avian Pathogenic E. coli*
**作者**: Wang L, et al.
**摘要**: 评估重组iutA蛋白作为疫苗的潜力，动物实验表明其可诱导高水平抗体，显著降低致病菌的感染率。
3. **标题**: *Structural Insights into iutA-mediated Iron Acquisition by X-ray Crystallography*
**作者**: Garcia R, et al.
**摘要**: 通过重组iutA蛋白的晶体结构解析，揭示了其与铁载体复合物的结合位点，为设计新型抗菌药物提供结构依据。
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以上文献方向涵盖基因表达、疫苗开发及结构解析，均围绕iutA重组蛋白的功能与应用展开。实际文献需通过学术数据库检索获取。
The iutA gene encodes a critical outer membrane receptor protein involved in iron acquisition in Gram-negative bacteria like *Escherichia coli*. Iron is essential for bacterial growth but is tightly sequestered by host proteins during infection, prompting pathogens to evolve sophisticated uptake systems. The iutA protein is a key component of the aerobactin-mediated iron transport system, enabling bacteria to scavenge iron by binding and internalizing iron-bound aerobactin, a siderophore secreted by the bacteria themselves. This system is particularly vital for pathogenic strains, such as uropathogenic *E. coli* (UPEC), where iron acquisition directly correlates with virulence and survival in iron-limited host environments.
Recombinant iutA protein is produced through genetic engineering, typically by cloning the iutA gene into expression vectors, followed by overexpression in bacterial hosts like *E. coli*. Purification involves techniques such as affinity chromatography, yielding a protein that retains its functional receptor properties. Studies on recombinant iutA have provided insights into its structural features, including receptor-ligand interactions and membrane localization mechanisms.
Research on iutA has significant implications for antimicrobial strategies. Inhibiting iutA-mediated iron uptake could attenuate bacterial virulence, making it a potential therapeutic target. Additionally, recombinant iutA serves as a tool for vaccine development, as antibodies targeting this protein may block iron acquisition and reduce infectivity. Its role in diagnostics is also explored, as iutA expression may serve as a marker for identifying highly virulent bacterial strains. Overall, iutA recombinant protein studies bridge fundamental microbiology and applied clinical research, offering avenues for novel infection control approaches.
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