| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | shf |
| Uniprot No | Q7M4L6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-423aa |
| 氨基酸序列 | MQQEGGPVRS APCRTGTLEG SRQGSPGHRK RASPKGSLSS AQPHSWMLTP SPLNSHCAHR EPISSSPQPV ANGPKQKKKS NWRSTTRLRI IRLRDRLEPR PLAILEDYAD PFDVQETGEG SAGASGAPEK VPENDGYMEP YEAQKMMAEI RGSKETATQP LPLYDTPYEP EEDGATAEGE GAPWPRESRL PEDDERPPEE YDQPWEWKKE RISKAFAVDI KVIKDLPWPP PVGQLDSSPS LPDGDRDISG PASPLPEPSL EDSSAQFEGP EKSCLSPGRE EKGRLPPRLS AGNPKSAKPL SMEPSSPLGE WTDPALPLEN QVWYHGAISR TDAENLLRLC KEASYLVRNS ETSKNDFSLS LKSSQGFMHM KLSRTKEHKY VLGQNSPPFS SVPEIVHHYA SRKLPIKGAE HMSLLYPVAI RTL |
| 预测分子量 | 46,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SHF重组蛋白的假设性参考文献示例(实际文献需根据具体研究方向检索)：
1. **文献名称**：Structural Characterization of SHF Recombinant Protein in Cancer Signaling
**作者**：Li et al. (2022)
**摘要**：研究通过X射线晶体学解析了SHF重组蛋白的三维结构，发现其通过特定结构域与肿瘤信号通路关键分子相互作用，为靶向治疗提供了结构基础。
2. **文献名称**：High-Yield Expression and Purification of SHF Protein in E. coli
**作者**：Zhang & Wang (2020)
**摘要**：优化了SHF重组蛋白在大肠杆菌中的表达条件，采用亲和层析技术实现高纯度制备，为体外功能研究提供了可靠方法。
3. **文献名称**：SHF Recombinant Protein Modulates Neurodegenerative Pathways in vitro
**作者**：Kim et al. (2021)
**摘要**：体外实验表明，SHF蛋白通过调节tau蛋白磷酸化水平影响神经细胞存活，提示其在阿尔茨海默病中的潜在作用。
4. **文献名称**：Functional Analysis of SHF Domains in Viral Entry Inhibition
**作者**：Smith et al. (2019)
**摘要**：通过截断体实验验证SHF重组蛋白的特定结构域可阻断病毒与宿主细胞膜融合，为抗病毒药物开发提供新靶点。
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**说明**：
- SHF重组蛋白的研究方向可能涉及结构解析、表达优化、疾病机制或药物开发等领域。
- 实际文献建议通过PubMed/Google Scholar以关键词“SHF recombinant protein” + 研究领域(如“cancer”“purification”)检索。
- 若SHF为特定蛋白缩写(如SH3 domain fusion)，需调整检索策略。
**Background of SHF Recombinant Proteins**
Recombinant proteins, engineered through genetic modification, are pivotal in modern biotechnology and biomedical research. SHF (synthetic hybrid fusion) recombinant proteins represent a specialized class designed to enhance functionality, stability, or targeted delivery. These proteins are typically constructed by fusing functional domains—such as binding motifs, enzymatic regions, or structural elements—from distinct parent proteins. This modular approach allows researchers to tailor proteins for specific applications, including therapeutics, diagnostics, and industrial biocatalysis.
The development of SHF recombinant proteins leverages advances in molecular biology, such as codon optimization, fusion tag systems (e.g., His-tags, Fc regions), and high-yield expression platforms (e.g., *E. coli*, yeast, or mammalian cell cultures*). For instance, SHF proteins often incorporate Fc domains to prolong serum half-life or solubility-enhancing tags to improve production efficiency. Their design may also address challenges like immunogenicity or aggregation, common in wild-type proteins.
In therapeutics, SHF recombinant proteins are explored for targeted drug delivery, immunotherapy, and vaccine development. Examples include bispecific antibodies or cytokine fusion proteins that simultaneously engage multiple disease targets. In diagnostics, they serve as highly specific detection probes or biosensors. Industrial applications range from enzyme cascades for biofuel production to engineered proteases in sustainable manufacturing.
Despite their promise, SHF protein development faces hurdles, including structural instability during fusion, unintended interactions, and scalability issues. Ongoing research focuses on computational modeling (e.g., AI-driven protein design) and novel expression systems to refine their precision and applicability. As tools like CRISPR and high-throughput screening evolve, SHF recombinant proteins are poised to play an expanding role in addressing complex biomedical and industrial challenges.
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