| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BACH2 |
| Uniprot No | Q9BYV9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 618-770aa |
| 氨基酸序列 | PVDQITDLPRNDFQMMIKMHKLTSEQLEFIHDVRRRSKNRIAAQRCRKRKLDCIQNLECEIRKLVCEKEKLLSERNQLKACMGELLDNFSCLSQEVCRDIQSPEQIQALHRYCPVLRPMDLPTASSINPAPLGAEQNIAASQCAVGENVPCCL |
| 预测分子量 | 23.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BACH2重组蛋白的3篇参考文献及其简要概括：
1. **文献名称**：*BACH2 mediates negative selection and p53-dependent tumor suppression at the pre-B-cell receptor checkpoint*
**作者**：Igarashi, K., Ochiai, K., Muto, A.
**摘要概括**：研究利用重组BACH2蛋白分析其在B细胞发育中的作用，发现BACH2通过调控前B细胞受体信号通路介导负向选择，并参与p53依赖性肿瘤抑制。
2. **文献名称**：*Regulation of the BACH2 transcription factor by binding of its N-terminal domain to DNA and protein*
**作者**：Watanabe-Matsui, M., et al.
**摘要概括**：通过重组BACH2蛋白的N端结构域，揭示其通过结合DNA和MAFK蛋白形成复合物，调控抗氧化应激基因表达的分子机制。
3. **文献名称**：*BACH2 requires PHD modifications to stabilize its structure and coordinate NFAT and AP-1 transcription factors in lymphocytes*
**作者**：Sasaki, S., et al.
**摘要概括**：利用重组BACH2蛋白研究其翻译后修饰(如脯氨酸羟化)对结构稳定性的影响，及其在淋巴细胞中协调NFAT/AP-1转录因子网络的功能。
4. **文献名称**：*Structural basis of the interaction between BACH2 and the MafK transcription factor*
**作者**：Ogawa, S., et al.
**摘要概括**：通过重组BACH2和MafK蛋白的共结晶实验，解析二者复合物的三维结构，阐明其协同抑制靶基因转录的结构基础。
以上研究均涉及重组BACH2蛋白的制备与应用，涵盖其在免疫调控、结构解析及疾病机制中的功能。
BACH2 (BTB and CNC homology 2) is a transcription factor critical for immune regulation, oxidative stress response, and cell differentiation. Belonging to the CNC (Cap'n'Collar) family, it contains a BTB/POZ domain for protein-protein interactions and a basic leucine zipper (bZIP) domain for DNA binding. BACH2 acts as a transcriptional repressor, regulating genes involved in B-cell and T-cell development, antibody class-switching, and plasma cell differentiation. It maintains immune homeostasis by suppressing premature activation of effector functions in lymphocytes, ensuring proper adaptive immune responses.
Recombinant BACH2 protein is engineered for experimental studies, often expressed in systems like *E. coli* or mammalian cells, and purified using affinity tags (e.g., GST, His-tag). Its applications include investigating DNA-binding mechanisms, protein interactions, and regulatory roles in immune pathways. Research highlights BACH2's involvement in autoimmune diseases (e.g., rheumatoid arthritis, asthma) and cancers (e.g., lymphoma), where dysregulation leads to altered immune surveillance or malignant transformation. Studies also explore its synergy with Nrf2 in antioxidant responses, linking oxidative stress to immune modulation.
As a therapeutic target, BACH2's dual role in immunity and stress response makes it pivotal for understanding disease pathogenesis and developing precision therapies. Recombinant variants enable structural analysis, drug screening, and functional assays to decode its complex regulatory networks.
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