| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Entrez GeneID | 51560 |
| clone | 6D12G9 |
| WB Predicted band size | 23.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Purified recombinant fragment of human Rab6b (AA: 95-208) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于Rab6b抗体的3篇代表性文献(均为示例，非真实文献)：
1. **文献名称**：**"Rab6b regulates Golgi-to-ER retrograde transport through interaction with COPI machinery"**
**作者**：Smith A, et al.
**摘要**：本研究利用特异性Rab6b抗体，通过免疫荧光和免疫共沉淀技术，揭示了Rab6b在调控高尔基体至内质网逆向运输中的作用，并证实其与COPI复合物的相互作用对囊泡形成至关重要。
2. **文献名称**：**"Characterization of a novel Rab6b monoclonal antibody for neuronal trafficking studies"**
**作者**：Chen L, et al.
**摘要**：文章报道了一种新型Rab6b单克隆抗体的开发与验证，该抗体在Western blot和免疫组化中表现出高特异性，并成功应用于神经元内运输机制的研究，显示Rab6b在突触囊泡循环中的潜在功能。
3. **文献名称**：**"Dysregulation of Rab6b in colorectal cancer: Implications for tumor progression"**
**作者**：Wang Y, et al.
**摘要**：通过Rab6b抗体分析结直肠癌组织样本，发现Rab6b表达水平与肿瘤侵袭性正相关，实验表明Rab6b通过调控Wnt信号通路影响癌细胞迁移和转移。
(注：以上文献为模拟内容，实际研究中请通过PubMed或Google Scholar查询真实文献。)
The Rab6b antibody is a crucial tool for studying the Rab6b protein, a member of the Rab GTPase family involved in intracellular membrane trafficking. Rab6b, an isoform of Rab6. regulates retrograde transport from the Golgi apparatus to the endoplasmic reticulum (ER) and participates in secretory pathways, autophagy, and cell division. Unlike Rab6a, Rab6b exhibits distinct expression patterns, enriched in neuronal and endocrine tissues, suggesting specialized roles in secretory processes or neurotransmitter release.
Rab6b antibodies are typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant proteins. These antibodies enable detection and localization of Rab6b via techniques such as Western blotting, immunofluorescence, and immunoprecipitation. Specificity validation—through knockdown/knockout controls or isoform cross-reactivity tests—is critical due to high homology within the Rab family.
Research applications include exploring Rab6b's involvement in diseases like neurodegenerative disorders (e.g., impaired vesicle trafficking in Alzheimer’s) and cancers (e.g., dysregulated secretion in tumors). Commercial antibodies often cite peer-reviewed studies, but performance may vary depending on experimental conditions (e.g., fixation methods or tissue types). Recent studies also highlight Rab6b's interaction with motor proteins (e.g., dynein) and tethering complexes, emphasizing its role in maintaining organelle architecture.
In summary, Rab6b antibodies are vital for dissecting the protein's trafficking mechanisms and disease associations, requiring careful validation to ensure precise experimental outcomes.
×
