| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | Rh2b |
| Uniprot No | C0H5F4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1800-2000aa |
| 氨基酸序列 | VKKQYIKTIEDVKFLLDSLNTIEEKNKSVANLEICTNKEDIKNLLKHVIKLANFSGIIVMSDTNTEITPENPLEDNDLLNLQLYFERKHEITSTLENDSDLELDHLGSNSDESIDNLKVYNDIIELHTYSTQILKYLDNIQKLKGDCNDLVKDCKELRELSTALYDLKIQITSVINRENDISNNIDIVSNKLNEIDAIQYN |
| 预测分子量 | 23.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于Rh2b重组蛋白的参考文献示例(仅供参考，实际文献需通过学术数据库验证)：
1. **文献名称**：*Expression and spectral tuning mechanism of zebrafish Rh2b cone pigment*
**作者**：Chinen A, et al.
**摘要**：本研究克隆了斑马鱼视锥细胞中的Rh2b视蛋白基因，通过重组表达于HEK293细胞，测定其吸收光谱特性，发现其最大吸收波长与体内特性一致，揭示了Rh2b光敏感性的分子机制。
2. **文献名称**：*Functional characterization of recombinant human GABAρ2b receptor subunits*
**作者**：Wang Y, et al.
**摘要**：构建了人源GABA-A受体rho2b亚基的重组表达系统，通过电生理实验证实其形成同源五聚体通道，并对配体结合特性进行分析，为神经递质受体研究提供模型。
3. **文献名称**：*Rh2b chemokine receptor signaling in inflammatory responses*
**作者**：Smith JL, et al.
**摘要**：利用重组Rh2b蛋白研究其与趋化因子的相互作用，发现其在白细胞迁移中的关键作用，并通过体外实验验证了特定抑制剂对信号通路的调控。
4. **文献名称**：*Optimized production of Rh2b fusion protein in E. coli for antibody development*
**作者**：Kim H, et al.
**摘要**：报道了一种高效表达Rh2b重组蛋白的大肠杆菌系统，通过密码子优化和纯化策略改进，获得高纯度蛋白用于单克隆抗体制备及疾病诊断应用。
**注意**：以上内容为模拟示例，Rh2b的生物学背景需结合具体研究领域(如视觉蛋白、受体亚基等)。建议通过**PubMed/Google Scholar**以关键词“Rh2b recombinant”或“Rh2b + 具体功能”检索真实文献，或进一步确认目标蛋白的全称及研究背景。
**Background of Rh2b Recombinant Protein**
The Rh2b recombinant protein is a genetically engineered biomolecule derived from the Rh2b gene, which encodes a protein involved in critical cellular processes such as signal transduction, immune regulation, or metabolic pathways, depending on its biological origin. Recombinant Rh2b is typically produced using heterologous expression systems, such as *E. coli*, yeast, or mammalian cell lines, to ensure high yield and purity. The gene encoding Rh2b is cloned into an expression vector, followed by transfection into a host organism, where it is expressed and subsequently purified via chromatography techniques like affinity or size-exclusion methods.
Rh2b’s significance stems from its structural or functional role in specific biological contexts. For instance, if Rh2b is a cytokine or receptor, it may modulate immune responses; if it is an enzyme, it could catalyze metabolic reactions. Its recombinant form allows for precise study of its mechanisms, post-translational modifications, and interactions with other biomolecules under controlled conditions.
Applications of Rh2b recombinant protein span basic research, diagnostics, and therapeutics. In research, it serves as a tool for elucidating pathways in diseases like cancer or autoimmune disorders. In diagnostics, it may act as an antigen or antibody in assay development. Therapeutically, Rh2b could be engineered for enhanced stability or targeting, potentially serving as a biologic drug or vaccine component.
Quality control, including SDS-PAGE, Western blotting, and functional assays, ensures batch consistency and activity. Challenges in production include optimizing expression conditions and minimizing host-derived contaminants. Ongoing studies focus on improving its bioactivity and exploring novel applications in biotechnology and medicine.
In summary, Rh2b recombinant protein represents a versatile tool bridging molecular biology and applied sciences, with broad potential in understanding and manipulating biological systems.
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