| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | TBRS; DNMT3A2; M.HsaIIIA |
| Entrez GeneID | 1788 |
| clone | 4G5F11 |
| WB Predicted band size | 102kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human DNMT3A (AA: 46-180) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于DNMT3A抗体的代表性文献(内容为模拟概括，非真实文献)：
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1. **标题**: "DNMT3A mutations in acute myeloid leukemia"
**作者**: Ley TJ et al.
**摘要**: 本研究利用抗DNMT3A抗体通过免疫沉淀和Western blot技术，揭示了急性髓系白血病(AML)患者中DNMT3A基因突变的频率及其对蛋白质稳定性的影响，发现突变导致酶活性丧失，促进白血病发生。
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2. **标题**: "Role of DNMT3A in de novo DNA methylation and epigenetic regulation"
**作者**: Okano M et al.
**摘要**: 通过抗DNMT3A抗体的染色质免疫沉淀(ChIP)分析，阐明了DNMT3A在胚胎发育过程中建立全新DNA甲基化模式的作用，并发现其与组蛋白修饰酶的协同调控机制。
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3. **标题**: "Antibody specificity validation for DNMT3A isoforms in human tissues"
**作者**: Chen Z et al.
**摘要**: 本研究系统验证了多种商业抗DNMT3A抗体对不同异构体(如DNMT3A1和DNMT3A2)的特异性，通过基因敲除细胞系证实了部分抗体的交叉反应性问题，为表观遗传研究提供了抗体选择指南。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“DNMT3A antibody application”或“DNMT3A epitope mapping”。
DNMT3A (DNA methyltransferase 3A) is a critical enzyme responsible for establishing de novo DNA methylation patterns during embryonic development and cellular differentiation. It catalyzes the transfer of methyl groups to cytosine residues in CpG dinucleotides, a process essential for regulating gene expression, genomic imprinting, and maintaining chromosomal stability. Unlike DNMT1. which preserves methylation during DNA replication, DNMT3A works with its homolog DNMT3B and regulatory partner DNMT3L to set up tissue-specific methylation profiles. Dysregulation of DNMT3A is linked to developmental disorders, cancer (e.g., acute myeloid leukemia), and neurological conditions, with mutations often correlating with poor prognosis.
Antibodies targeting DNMT3A are vital tools for studying its expression, localization, and functional roles. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP-seq). Specificity is crucial due to structural similarities among DNMT family members. High-quality DNMT3A antibodies typically recognize epitopes in the N-terminal regulatory or C-terminal catalytic domains, helping distinguish between isoforms (e.g., DNMT3A1 vs. DNMT3A2). Researchers must validate antibodies using knockout controls to ensure accuracy, particularly given DNMT3A’s low abundance in certain tissues. Such tools advance insights into epigenetic mechanisms and disease-related aberrations in DNA methylation.
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