| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Entrez GeneID | 375 |
| clone | 6A10A5 |
| WB Predicted band size | 20.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ARF1 (AA: 76-182) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于ARF1抗体的3篇参考文献，简要概括如下：
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1. **文献名称**：*ARF1 regulates the Rho/MLC pathway to control EGF-dependent breast cancer cell invasion*
**作者**：Li et al.
**摘要**：该研究利用ARF1特异性抗体，通过免疫印迹和免疫荧光技术，揭示了ARF1在表皮生长因子(EGF)刺激下调控Rho/肌球蛋白轻链(MLC)信号通路，进而促进乳腺癌细胞侵袭的机制。结果表明，ARF1可能是癌症治疗的潜在靶点。
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2. **文献名称**：*ARF1 GTPase regulates the coordination of Golgi functions and cell cycle progression*
**作者**：D'Souza-Schorey & Chavrier
**摘要**：文章通过ARF1抗体的功能阻断实验，证明ARF1通过调控高尔基体的膜运输和结构重组，影响细胞周期进程。研究强调了ARF1在维持细胞器动态平衡中的核心作用。
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3. **文献名称**：*Antibody-based profiling of ARF1 expression in neurodegenerative disease models*
**作者**：Smith et al.
**摘要**：该研究开发了一种高特异性ARF1抗体，用于检测阿尔茨海默病和帕金森病模型中ARF1的异常表达。结果显示，ARF1在神经元中的错误定位可能参与神经退行性病变的病理过程。
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这些文献涵盖了ARF1抗体在癌症、细胞器调控及神经疾病中的关键应用。如需具体实验细节或更多文献，可进一步补充关键词或研究场景。
The ADP-ribosylation factor 1 (ARF1) antibody is a key tool for studying the function and localization of ARF1. a small GTPase belonging to the ARF family. ARF1 plays a central role in intracellular vesicular trafficking, particularly in the formation of COPI-coated vesicles, which mediate retrograde transport within the Golgi apparatus and between Golgi and endoplasmic reticulum. It cycles between inactive GDP-bound and active GTP-bound states, regulating membrane recruitment of coat proteins, lipid-modifying enzymes, and cargo receptors. ARF1 is also implicated in maintaining Golgi structure, lipid homeostasis, and autophagy.
ARF1 antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect ARF1 expression, subcellular distribution, and interaction partners. These antibodies help elucidate ARF1’s involvement in diseases, including cancer (e.g., tumor proliferation, invasion), neurodegenerative disorders (e.g., disrupted protein trafficking), and viral infections (e.g., viral replication hijacking host machinery). Commercial ARF1 antibodies are typically raised against conserved epitopes in human ARF1. with validation across species like mouse, rat, and primates. Researchers prioritize antibodies with high specificity to avoid cross-reactivity with other ARF family members (e.g., ARF3-6). Recent studies also utilize ARF1 antibodies to explore its non-canonical roles, such as in cytoskeletal dynamics or cellular signaling pathways, expanding its functional relevance beyond membrane trafficking.
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