| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | nuc1 |
| Uniprot No | Q03181 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 165-441aa |
| 氨基酸序列 | GSQYNPQVADLKAFSKHIYNAYLKNFNMTKKKARSILTGKASHTAPFVIHDIETLWQAEKGLVWKQLVNGLPPYKEISVHVFYRCQCTTVETVRELTEFAKSIPSFSSLFLNDQVTLLKYGVHEAIFAMLASIVNKDGLLVANGSGFVTREFLRSLRKPFSDIIEPKFEFAVKFNALELDDSDLALFIAAIILCGDRPGLMNVPRVEAIQDTILRALEFHLQANHPDAQYLFPKLLQKMADLRQLVTEHAQMMQRIKKTETETSLHPLLQEIYKDMY |
| 预测分子量 | 49.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Nuc1重组蛋白的3-4篇参考文献的简要整理(文献标题及内容为模拟示例)：
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1. **标题**：*"Cloning, Expression, and Functional Characterization of Recombinant Nuc1 from Staphylococcus aureus*"
**作者**：Smith A, et al.
**摘要**：本研究成功克隆并表达了金黄色葡萄球菌Nuc1核酸酶的重组形式，通过大肠杆菌系统纯化获得高活性蛋白。实验表明，重组Nuc1能有效降解细菌生物膜中的胞外DNA，并增强抗生素对耐药菌的渗透性，提示其作为抗菌辅助剂的潜力。
2. **标题**：*"Structural Insights into the Catalytic Mechanism of Recombinant Nuc1 Protein*"
**作者**：Johnson R, et al.
**摘要**：通过X射线晶体学解析了重组Nuc1的三维结构，揭示了其依赖钙离子的DNA水解活性位点，并发现突变关键氨基酸残基会显著降低酶活性，为设计靶向抑制剂提供了结构基础。
3. **标题**：*"Application of Recombinant Nuc1 in Targeted Drug Delivery Systems*"
**作者**：Brown L, et al.
**摘要**：利用重组Nuc1的DNA结合特性，开发了一种基于该蛋白的纳米药物载体，可定向递送化疗药物至肿瘤细胞。体外实验显示，该系统显著提高了药物在DNA富集区域的靶向性和释放效率。
4. **标题**：*"Role of Recombinant Nuc1 in Modulating Biofilm Formation and Immune Response*"
**作者**：Lee C, et al.
**摘要**：研究发现，重组Nuc1通过降解中性粒细胞胞外陷阱(NETs)减轻过度炎症反应，同时抑制病原菌生物膜形成，表明其在治疗慢性感染和炎症性疾病中的双重作用。
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**注**：以上文献为示例，实际研究中请通过PubMed、Web of Science等平台检索具体论文。如需真实文献，可进一步提供研究方向(如抗菌、结构或应用)以便精准推荐。
Here’s a concise overview of Nuc1 recombinant protein:
Nuc1 (Nuclease 1) is a bacterially derived endonuclease initially characterized in *Staphylococcus aureus*, where it functions as a thermostable enzyme capable of degrading both DNA and RNA. As a recombinant protein, it has been engineered for biotechnological applications through heterologous expression systems (typically *E. coli*), enabling large-scale production with purification tags like His-tag for simplified isolation.
This 18.7 kDa enzyme exhibits unique biochemical properties, including optimal activity at neutral to alkaline pH (7.0-9.0) and dependence on Ca²⁺ ions for activation. Unlike many nucleases, Nuc1 maintains functionality at elevated temperatures (up to 60°C) while being inactivated through simple EDTA chelation or heat treatment (≥75°C), making it particularly useful in processes requiring controlled nucleic acid removal.
In bioproduction workflows, recombinant Nuc1 serves as an efficient tool for eliminating host cell nucleic acids during protein purification, vaccine manufacturing, and viral vector production. Its applications extend to molecular biology techniques such as PCR cleanup and next-generation sequencing library preparation, where residual nucleic acid removal is critical. Compared to alternatives like Benzonase®, Nuc1 demonstrates superior thermal stability and easier inactivation, reducing the risk of enzyme carryover in final products.
The commercial availability of recombinant Nuc1 has expanded its utility in pharmaceutical development and diagnostic kit manufacturing, particularly where regulatory compliance demands traceable, tag-free enzyme preparations. Recent studies also explore its potential in chromatin research and RNA-protein interaction analyses through controlled nucleic acid digestion protocols.
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