| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ORF11 |
| Uniprot No | Q9UHD2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-729aa |
| 氨基酸序列 | MQSTSNHLWLLSDILGQGATANVFRGRHKKTGDLFAIKVFNNISFLRPVDVQMREFEVLKKLNHKNIVKLFAIEEETTTRHKVLIMEFCPCGSLYTVLEEPSNAYGLPESEFLIVLRDVVGGMNHLRENGIVHRDIKPGNIMRVIGEDGQSVYKLTDFGAARELEDDEQFVSLYGTEEYLHPDMYERAVLRKDHQKKYGATVDLWSIGVTFYHAATGSLPFRPFEGPRRNKEVMYKIITGKPSGAISGVQKAENGPIDWSGDMPVSCSLSRGLQVLLTPVLANILEADQEKCWGFDQFFAETSDILHRMVIHVFSLQQMTAHKIYIHSYNTATIFHELVYKQTKIISSNQELIYEGRRLVLEPGRLAQHFPKTTEENPIFVVSREPLNTIGLIYEKISLPKVHPRYDLDGDASMAKAITGVVCYACRIASTLLLYQELMRKGIRWLIELIKDDYNETVHKKTEVVITLDFCIRNIEKTVKVYEKLMKINLEAAELGEISDIHTKLLRLSSSQGTIETSLQDIDSRLSPGGSLADAWAHQEGTHPKDRNVEKLQVLLNCMTEIYYQFKKDKAERRLAYNEEQIHKFDKQKLYYHATKAMTHFTDECVKKYEAFLNKSEEWIRKMLHLRKQLLSLTNQCFDIEEEVSKYQEYTNELQETLPQKMFTASSGIKHTMTPIYPSSNTLVEMTLGMKKLKEEMEGVVKELAENNHILERFGSLTMDGGLRNVDCL |
| 预测分子量 | 83,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于已有知识的模拟参考文献示例，供参考使用(注：实际文献可能需要通过学术数据库验证)：
1. **文献名称**: "Characterization of KSHV ORF11 Recombinant Protein and Its Role in Immune Evasion"
**作者**: Smith A, et al.
**摘要**: 本研究在大肠杆菌中表达了KSHV的ORF11重组蛋白，发现其通过抑制干扰素信号通路阻碍宿主先天免疫应答。实验表明ORF11与STAT2蛋白相互作用，可能为病毒免疫逃逸机制的关键因子。
2. **文献名称**: "Structural Analysis of ORF11 Recombinant Protein from Murine Gammaherpesvirus 68"
**作者**: Lee C, et al.
**摘要**: 通过X射线晶体学解析了MHV-68 ORF11重组蛋白的三维结构，揭示了其独特的α-螺旋结构域，并推测其可能与宿主细胞膜融合过程相关，为抗病毒药物设计提供结构基础。
3. **文献名称**: "Functional Screening of ORF11 Recombinant Protein in Viral DNA Replication"
**作者**: Gupta R, et al.
**摘要**: 利用杆状病毒系统表达ORF11重组蛋白，发现其与病毒DNA聚合酶共定位，体外实验证实ORF11可增强病毒基因组复制效率，提示其在病毒生命周期中的直接作用。
4. **文献名称**: "ORF11 Recombinant Protein as a Novel Diagnostic Antigen for Herpesvirus-associated Diseases"
**作者**: Zhang Y, et al.
**摘要**: 评估了ORF11重组蛋白作为血清学诊断标志物的潜力，ELISA显示其在患者样本中具有高特异性，可能用于卡波西肉瘤的早期检测。
**建议**：实际研究中请通过PubMed、Web of Science等平台以“ORF11 recombinant protein”及病毒名称(如KSHV、MHV-68等)为关键词检索最新文献，并优先选择高影响因子期刊论文。
**Background of ORF11 Recombinant Protein**
ORF11 recombinant protein is derived from the open reading frame 11 (ORF11) gene, which is commonly associated with viral genomes, particularly herpesviruses such as Varicella-Zoster virus (VZV) or Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8). ORF11 encodes a conserved protein implicated in viral replication, immune evasion, or host-cell interactions, though its precise functional mechanisms remain under investigation. In VZV, ORF11 is a tegument protein suggested to play roles in viral assembly, egress, or modulation of host immune responses, potentially interfering with interferon signaling pathways.
Recombinant ORF11 protein is engineered using heterologous expression systems (e.g., *E. coli*, insect, or mammalian cells*) to produce purified protein for functional and structural studies. Its production enables researchers to study antigenicity, protein-protein interactions, and immune responses, aiding vaccine development or diagnostic tool design. For instance, ORF11-specific antibodies may serve as biomarkers for viral infection or targets for therapeutic intervention.
Research on ORF11 also extends to understanding its role in viral pathogenesis. In KSHV, ORF11 homologs are linked to viral latency and reactivation, influencing oncogenic pathways. Structural characterization of the recombinant protein (e.g., via crystallography or cryo-EM) provides insights into its functional domains and potential drug-binding sites.
Challenges include optimizing expression conditions to ensure proper folding and post-translational modifications, which are critical for functional studies. Despite these hurdles, ORF11 recombinant protein remains a valuable tool for unraveling viral biology and advancing antiviral strategies. Ongoing studies aim to clarify its interactions with host proteins and its contribution to viral lifecycle stages, offering avenues for novel therapeutics.
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