| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KCNK13 |
| Uniprot No | Q9HB14 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-408aa |
| 氨基酸序列 | MAGRGFSWGPGHLNEDNARFLLLAALIVLYLLGGAAVFSALELAHERQAKQRWEERLANFSRGHNLSRDELRGFLRHYEEATRAGIRVDNVRPRWDFTGAFYFVGTVVSTIGFGMTTPATVGGKIFLIFYGLVGCSSTILFFNLFLERLITIIAYIMKSCHQRQLRRRGALPQESLKDAGQCEVDSLAGWKPSVYYVMLILCTASILISCCASAMYTPIEGWSYFDSLYFCFVAFSTIGFGDLVSSQNAHYESQGLYRFANFVFILMGVCCIYSLFNVISILIKQSLNWILRKMDSGCCPQCQRGLLRSRRNVVMPGSVRNRCNISIETDGVAESDTDGRRLSGEMISMKDLLAANKASLAILQKQLSEMANGCPHQTSTLARDNEFSGGVGAFAIMNNRLAETSGDR |
| 预测分子量 | 51.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KCNK13重组蛋白的3篇代表性文献(信息基于公开研究内容概括)：
1. **"KCNK13: A novel two-pore domain potassium channel in human cancers"**
- **作者**: Smith A, et al.
- **摘要**: 研究揭示了KCNK13(TASK-10)在多种癌症中的异常表达，利用重组蛋白模型证明其通过调控细胞膜电位影响肿瘤细胞迁移和侵袭。
2. **"Functional characterization of recombinant KCNK13 channels in Xenopus oocytes"**
- **作者**: Lee JH, et al.
- **摘要**: 通过非洲爪蟾卵母细胞系统表达重组KCNK13蛋白，分析其电生理特性及pH/机械力敏感性，揭示其在维持细胞稳态中的作用。
3. **"KCNK13 as a potential drug target for neuropathic pain"**
- **作者**: Chen R, et al.
- **摘要**: 利用重组KCNK13蛋白筛选小分子抑制剂，发现其调控神经元兴奋性的机制，为开发新型镇痛药物提供理论依据。
(注：以上内容为模拟概括，实际文献需通过PubMed或Google Scholar检索确认。)
**Background of KCNK13 Recombinant Protein**
KCNK13. also known as potassium channel subfamily K member 13. is a member of the two-pore domain potassium (K2P) channel family. These channels play critical roles in maintaining cellular resting membrane potential and regulating excitability in various tissues. KCNK13 is distinguished by its unique structural features, including four transmembrane domains and two pore-forming loops, which enable selective potassium ion conduction. It functions as a "leak" channel, contributing to background potassium currents that stabilize membrane voltage and modulate cellular responses to physiological stimuli.
The KCNK13 gene is expressed in multiple tissues, including the pancreas, brain, and cardiovascular system. Studies suggest its involvement in insulin secretion from pancreatic β-cells, neuronal signaling, and vascular tone regulation. Dysregulation of KCNK13 has been linked to metabolic disorders such as diabetes, neurological conditions, and hypertension. For instance, gain-of-function mutations in KCNK13 are associated with impaired glucose-stimulated insulin secretion, highlighting its potential as a therapeutic target.
Recombinant KCNK13 protein is produced using heterologous expression systems (e.g., HEK293 cells) to study its biophysical properties, regulatory mechanisms, and interactions with drugs or modulators. This engineered protein retains functional characteristics of the native channel, enabling electrophysiological analyses (e.g., patch-clamp) and high-throughput screening for channel activators or inhibitors. Researchers also utilize KCNK13 recombinant constructs to investigate disease-associated mutations or to develop targeted therapies.
Overall, KCNK13 recombinant protein serves as a vital tool for unraveling the channel’s physiological roles and its implications in human diseases, bridging molecular insights to potential clinical applications.
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