| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | fat-1 |
| Uniprot No | Q14517 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 4203-4588aa |
| 氨基酸序列 | RKMISRKKKHQAEPKDKHLGPATAFLQRPYFDSKLNKNIYSDIPPQVPVRPISYTPSIPSDSRNNLDRNSFEGSAIPEHPEFSTFNPESVHGHRKAVAVCSVAPNLPPPPPSNSPSDSDSIQKPSWDFDYDTKVVDLDPCLSKKPLEEKPSQPYSARESLSEVQSLSSFQSESCDDNGYHWDTSDWMPSVPLPDIQEFPNYEVIDEQTPLYSADPNAIDTDYYPGGYDIESDFPPPPEDFPAADELPPLPPEFSNQFESIHPPRDMPAAGSLGSSSRNRQRFNLNQYLPNFYPLDMSEPQTKGTGENSTCREPHAPYPPGYQRHFEAPAVESMPMSVYASTASCSDVSACCEVESEVMMSDYESGDDGHFEEVTIPPLDSQQHTEV |
| 预测分子量 | 49.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于fat-1重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*Cloning of fat-1 from Caenorhabditis elegans and its use in the omega-3 fatty acid biosynthesis*
**作者**：Kang, J.X., Wang, J., Wu, L., Kang, Z.B.
**摘要**：该研究首次克隆了线虫中的fat-1基因，并在哺乳动物细胞中成功表达。实验表明，重组fat-1蛋白能将细胞内的ω-6脂肪酸高效转化为ω-3脂肪酸，为生产富含ω-3的转基因动物奠定了基础。
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2. **文献名称**：*Generation of cloned transgenic pigs rich in omega-3 fatty acids*
**作者**：Lai, L., Kang, J.X., Li, R., et al.
**摘要**：研究团队将fat-1基因转入猪胎儿成纤维细胞，通过体细胞核移植技术获得转基因猪。这些猪的肌肉组织中ω-6/ω-3比例显著降低，证明了fat-1重组蛋白在大型哺乳动物中的功能性应用。
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3. **文献名称**：*Functional characterization of a recombinant Caenorhabditis elegans fatty acid desaturase (fat-1) in Saccharomyces cerevisiae*
**作者**：Pereira, S.L., Leonard, A.E., Mukerji, P.
**摘要**：本研究在酵母中异源表达fat-1重组蛋白，证实其具有Δ15去饱和酶活性，能将ω-6脂肪酸转化为ω-3.通过发酵优化，显著提高了酵母中ω-3脂肪酸的产量，为微生物合成提供了新策略。
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**备注**：以上文献发表于2000年代初期至中期，均为fat-1基因功能验证及应用的经典研究。如需最新进展，建议在PubMed或Web of Science中以“fat-1 recombinant protein”为关键词检索近年文献。
The fat-1 gene, originally identified in the soil nematode *Caenorhabditis elegans*, encodes an omega-3 fatty acid desaturase enzyme that catalyzes the conversion of omega-6 to omega-3 polyunsaturated fatty acids (PUFAs). This enzymatic activity is unique because mammals, including humans, lack endogenous fat-1-like enzymes and thus rely entirely on dietary intake to maintain omega-3 levels. The discovery of fat-1 in the early 2000s sparked significant interest in metabolic engineering, as its expression in non-native hosts could enable endogenous omega-3 production, addressing nutritional imbalances linked to modern diets rich in omega-6 but deficient in omega-3.
Researchers engineered transgenic animals (e.g., mice, pigs, cattle) and cell models expressing the fat-1 transgene to study its biological impacts. These models demonstrated reduced omega-6/omega-3 ratios in tissues, correlating with anti-inflammatory, cardioprotective, and neuroprotective benefits observed in human epidemiological studies. Fat-1 technology also offered potential applications in agriculture, such as producing omega-3-enriched meat, milk, or eggs without dietary supplementation. Additionally, it provided tools to investigate PUFA-mediated mechanisms in chronic diseases, cancer, and metabolic disorders.
Despite its promise, challenges persist, including public skepticism toward genetically modified organisms (GMOs), regulatory hurdles, and incomplete understanding of long-term effects. Recent advances in gene-editing technologies (e.g., CRISPR) have renewed interest in refining fat-1 integration for precision and safety. Overall, fat-1 recombinant protein research bridges nutrition, biotechnology, and medicine, offering innovative strategies to improve human health and sustainable food production.
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