| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UGT2B17 |
| Uniprot No | O75795 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-530aa |
| 氨基酸序列 | GKVLVWPTEYSHWINMKTILEELVQRGHEVIVLTSSASILVNASKSSAIKLEVYPTSLTKNDLEDFFMKMFDRWTYSISKNTFWSYFSQLQELCWEYSDYNIKLCEDAVLNKKLMRKLQESKFDVLLADAVNPCGELLAELLNIPFLYSLRFSVGYTVEKNGGGFLFPPSYVPVVMSELSDQMIFMERIKNMIYMLYFDFWFQAYDLKKWDQFYSEVLGRPTTLFETMGKAEMWLIRTYWDFEFPRPFLPNVDFVGGLHCKPAKPLPKEMEEFVQSSGENGIVVFSLGSMISNMSEESANMIASALAQIPQKVLWRFDGKKPNTLGSNTRLYKWLPQNDLLGHPKTKAFITHGGTNGIYEAIYHGIPMVGIPLFADQHDNIAHMKAKGAALSVDIRTMSSRDLLNALKSVINDPIYKENIMKLSRIHHDQPVKPLDRAVFWIEFVMRHKGAKHLRVAAHNLTWIQYHSLDVIAFLLACVATMIFMITKCCLFCFRKLAKTGKKKKRD |
| 预测分子量 | 64.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UGT2B17重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：**"Functional characterization of the UDP-glucuronosyltransferase 2B17 gene variant expressed in human cell lines"**
**作者**：Lévesque E, Beaulieu M, Guillemette C
**摘要**：该研究在HEK293细胞中重组表达了UGT2B17蛋白，分析了其催化活性和底物特异性。结果表明，UGT2B17对睾酮和二氢睾酮的葡萄糖醛酸化具有高活性，并揭示了其在类固醇代谢中的关键作用。
2. **文献名称**：**"Recombinant expression and kinetic analysis of the steroid-metabolizing enzyme UGT2B17"**
**作者**：Jakobsson J, Rane A, Ekström L
**摘要**：通过杆状病毒-昆虫细胞系统表达UGT2B17重组蛋白，测定了其对多种雄激素的酶动力学参数。研究发现UGT2B17对DHT的亲和力显著高于其他UGT2B亚型，为个体化药物代谢差异提供了机制解释。
3. **文献名称**：**"Impact of UGT2B17 gene deletion on recombinant enzyme activity and implications for doping detection"**
**作者**：Schulze JJ, Lorentzon M, Ohlsson C
**摘要**：研究利用重组UGT2B17蛋白分析了基因缺失表型对睾酮代谢的影响。结果表明，UGT2B17缺失会导致尿中睾酮葡萄糖醛酸代谢物减少，这对兴奋剂检测中假阴性结果的判定具有重要参考价值。
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以上文献涵盖了UGT2B17重组蛋白的表达系统(如哺乳动物细胞、昆虫细胞)、酶动力学特性及其在代谢研究与实际应用中的意义。如需扩展，可进一步检索涉及该蛋白的药物相互作用或癌症相关代谢的研究。
UGT2B17 is a member of the UDP-glucuronosyltransferase (UGT) enzyme family, which plays a critical role in the detoxification and elimination of both endogenous compounds (e.g., hormones, bilirubin) and xenobiotics (e.g., drugs, environmental toxins). This phase II metabolic enzyme catalyzes the conjugation of glucuronic acid to lipophilic substrates, enhancing their water solubility for excretion. UGT2B17 is primarily expressed in the liver, gastrointestinal tract, and steroid-responsive tissues, with genetic polymorphisms and copy number variations (CNVs) contributing to interindividual differences in drug metabolism and disease susceptibility. Its substrates include androgens, corticosteroids, and certain pharmaceuticals, linking it to hormone-related cancers, osteoporosis, and drug efficacy/toxicity.
Recombinant UGT2B17 protein is engineered for in vitro studies to overcome challenges in isolating native enzymes from tissues. Produced via heterologous expression systems (e.g., Escherichia coli, insect, or mammalian cell lines), the recombinant form retains enzymatic activity while enabling scalable purification. Researchers utilize it to investigate substrate specificity, enzyme kinetics, and drug-drug interactions, particularly for compounds metabolized through glucuronidation. Its application extends to personalized medicine, as genetic variants (e.g., UGT2B17 deletion polymorphisms) significantly alter metabolic capacity, impacting therapeutic outcomes. Studies also explore its role in cancer progression, as altered UGT2B17 expression may influence hormone-driven malignancies. However, functional characterization remains complex due to overlapping substrate preferences with other UGT2B isoforms and tissue-specific regulation. Recombinant UGT2B17 provides a standardized tool to dissect these mechanisms, advancing drug development and precision medicine strategies.
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