| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WARS1 |
| Uniprot No | P23381 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-471aa |
| 氨基酸序列 | PNSEPASLLELFNSIATQGELVRSLKAGNASKDEIDSAVKMLVSLKMSYKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYDKLIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDAYENKKPFYLYTGRGPSSEAMHVGHLIPFIFTKWLQDVFNVPLVIQMTDDEKYLWKDLTLDQAYSYAVENAKDIIACGFDINKTFIFSDLDYMGMSSGFYKNVVKIQKHVTFNQVKGIFGFTDSDCIGKISFPAIQAAPSFSNSFPQIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGYPKPALLHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQIKTKVNKHAFSGGRDTIEEHRQFGGNCDVDVSFMYLTFFLEDDDKLEQIRKDYTSGAMLTGELKKALIEVLQPLIAEHQARRKEVTDEIVKEFMTPRKLSFDFQ |
| 预测分子量 | 56.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WARS1重组蛋白的3篇参考文献的简要总结(注：文献信息为模拟示例，仅供参考)：
---
1. **文献名称**：*"Recombinant human tryptophanyl-tRNA synthetase (WARS1) exhibits pro-inflammatory cytokine-like activities"*
**作者**：Kim et al.
**摘要**：该研究成功在大肠杆菌中表达并纯化了重组人WARS1蛋白，发现其可通过激活NF-κB信号通路诱导巨噬细胞分泌炎症因子(如TNF-α和IL-6)，提示WARS1在先天免疫反应中的潜在作用。
2. **文献名称**：*"Structural and functional analysis of the catalytic domain of WARS1"*
**作者**：Yang et al.
**摘要**：通过X射线晶体学解析了重组WARS1蛋白的催化结构域三维结构，揭示了其底物结合位点的关键氨基酸残基，并验证了其酶活性对细胞增殖的影响，为设计靶向抑制剂奠定基础。
3. **文献名称**：*"Secreted WARS1 promotes angiogenesis via VEGF receptor interaction"*
**作者**：Wakasugi & Schimmel
**摘要**：研究发现重组WARS1蛋白在特定应激条件下被分泌至胞外，通过结合血管内皮生长因子受体(VEGFR)促进血管生成，提示其在肿瘤微环境或组织修复中的功能。
---
**注**：若需真实文献，建议通过PubMed或Google Scholar检索关键词“WARS1 recombinant protein”或“tryptophanyl-tRNA synthetase recombinant”获取最新研究。
**Background of WARS1 Recombinant Protein**
WARS1 (Tryptophanyl-tRNA Synthetase 1) is a cytoplasmic enzyme encoded by the *WARS1* gene, primarily responsible for catalyzing the attachment of tryptophan to its cognate tRNA during protein synthesis. As a member of the aminoacyl-tRNA synthetase (ARS) family, WARS1 ensures translational fidelity and is essential for maintaining cellular homeostasis. Beyond its canonical role, WARS1 has gained attention for its non-catalytic functions, including involvement in immune regulation, angiogenesis, and stress responses. Structural studies reveal conserved domains, including a catalytic core and unique N-terminal extensions, which may mediate interactions with signaling molecules or other cellular components.
Dysregulation of WARS1 is linked to various diseases. Elevated expression has been observed in cancers, where it may promote tumor progression via angiogenesis or immune evasion. Mutations in *WARS1* are associated with neurological disorders, such as autosomal dominant hearing loss, and autoimmune conditions like diabetic retinopathy. Its dual role as an enzyme and signaling molecule makes it a potential therapeutic target.
Recombinant WARS1 protein, produced using expression systems like *E. coli* or mammalian cells, retains enzymatic activity and structural integrity, enabling functional studies. It is widely used to investigate mechanistic pathways, screen inhibitors, or develop diagnostic tools. Additionally, its immunomodulatory properties are explored for treating inflammatory diseases. Research on WARS1 recombinant protein continues to uncover its multifaceted roles in health and disease, bridging basic biology and translational applications.
×
