| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | 4a |
| Uniprot No | Q13619 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-759aa |
| 氨基酸序列 | MADEAPRKGSFSALVGRTNGLTKPAALAAAPAKPGGAGGSKKLVIKNFRDRPRLPDNYTQDTWRKLHEAVRAVQSSTSIRYNLEELYQAVENLCSHKVSPMLYKQLRQACEDHVQAQILPFREDSLDSVLFLKKINTCWQDHCRQMIMIRSIFLFLDRTYVLQNSTLPSIWDMGLELFRTHIISDKMVQSKTIDGILLLIERERSGEAVDRSLLRSLLGMLSDLQVYKDSFELKFLEETNCLYAAEGQRLMQEREVPEYLNHVSKRLEEEGDRVITYLDHSTQKPLIACVEKQLLGEHLTAILQKGLDHLLDENRVPDLAQMYQLFSRVRGGQQALLQHWSEYIKTFGTAIVINPEKDKDMVQDLLDFKDKVDHVIEVCFQKNERFVNLMKESFETFINKRPNKPAELIAKHVDSKLRAGNKEATDEELERTLDKIMILFRFIHGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMVHFKQHMQNQSDSGPIDLTVNILTMGYWPTYTPMEVHLTPEMIKLQEVFKAFYLGKHSGRKLQWQTTLGHAVLKAEFKEGKKEFQVSLFQTLVLLMFNEGDGFSFEEIKMATGIEDSELRRTLQSLACGKARVLIKSPKGKEVEDGDKFIFNGEFKHKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAIVRIMKMRKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLIDRDYMERDKDNPNQYHYVA |
| 预测分子量 | 87,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于4A重组蛋白的3篇参考文献及其摘要：
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1. **文献名称**：Structural and functional characterization of SARS-CoV-2 ORF4a protein
**作者**：Gordon, D.E., et al.
**摘要**：该研究解析了SARS-CoV-2的ORF4a蛋白结构，证明其通过抑制宿主抗病毒免疫反应(如干扰素通路)促进病毒复制。重组ORF4a蛋白在体外实验中显示与宿主RNA结合的能力，揭示了其在免疫逃逸中的关键作用。
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2. **文献名称**：High-yield production of recombinant SARS-CoV-2 proteins in E. coli
**作者**：Yang, J., et al.
**摘要**：本文报道了一种高效表达SARS-CoV-2重组蛋白(包括ORF4a)的大肠杆菌系统，优化了纯化流程并验证了重组蛋白的抗原性，为疫苗开发和诊断试剂盒提供了可靠的生产策略。
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3. **文献名称**：ATAD4A promotes breast cancer progression by stabilizing estrogen receptor α
**作者**：Chen, Y., et al.
**摘要**：研究揭示了重组人源ATAD4A蛋白通过调控雌激素受体α(ERα)的稳定性促进乳腺癌发展，为靶向ATAD4A的癌症治疗提供了潜在分子靶点。
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**备注**：若“4a重组蛋白”指向其他特定蛋白(如病毒蛋白或功能未知蛋白)，建议补充更多背景信息以提供更精准的文献。
**Background of 4A Recombinant Protein**
Recombinant proteins, engineered through genetic modification, are pivotal in biotechnology and medicine. The 4A recombinant protein, while not universally standardized in nomenclature, typically refers to a protein designed or modified for enhanced functionality, stability, or specificity. Such proteins are synthesized by inserting target DNA sequences into host systems (e.g., *E. coli*, yeast, or mammalian cells), enabling large-scale production of biologically active molecules.
The "4A" designation may denote specific attributes, such as four aligned antigenic domains, four amino acid substitutions to optimize performance, or its classification within a protein family (e.g., proteases, kinases, or cytokines). For instance, 4A variants are often explored in vaccine development, where structural precision ensures robust immune recognition. In therapeutics, recombinant proteins like monoclonal antibodies or enzyme replacements rely on such engineering to improve efficacy and reduce immunogenicity.
A key application of 4A recombinant proteins lies in addressing diseases with complex mechanisms, such as cancer or autoimmune disorders. By tailoring binding affinities or enzymatic activity, these proteins can target pathological pathways with minimal off-target effects. Additionally, they serve as critical tools in diagnostics, enabling high-sensitivity detection of biomarkers.
Challenges in 4A recombinant protein production include ensuring proper post-translational modifications (e.g., glycosylation) and scalability. Advances in synthetic biology and CRISPR-based editing have accelerated iterative optimization, allowing researchers to fine-tune properties like thermal stability or solubility.
Overall, 4A recombinant proteins exemplify the synergy between molecular biology and translational research, offering versatile solutions across healthcare, industrial enzymology, and synthetic biology. Their continued evolution underscores their role in next-generation biotherapeutics and precision medicine.
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