| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | mazF |
| Uniprot No | P0AE70 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-111aa |
| 氨基酸序列 | MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLIKAKINVLIG |
| 预测分子量 | 13.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **mazF重组蛋白** 的参考文献及简要摘要：
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1. **《Escherichia coli mazEF-mediated cell death is triggered by various stressful conditions》**
- **作者**: Hazan R., Sat B., Engelberg-Kulka H.
- **摘要**: 研究证明，重组mazF蛋白在大肠杆菌中表达可诱导程序性细胞死亡，该过程由多种环境压力(如DNA损伤、营养缺乏)激活。mazF通过切割特定mRNA序列抑制蛋白合成，揭示了其在细菌应激反应中的关键作用。
2. **《Crystal structure of the MazF mRNA interferase from Escherichia coli》**
- **作者**: Kamada K., Hanaoka F., Burley S.K.
- **摘要**: 通过X射线晶体学解析了大肠杆菌mazF重组蛋白的三维结构，揭示了其核糖核酸内切酶活性位点及mRNA底物识别机制，为设计靶向mazF的抗菌药物提供了结构基础。
3. **《MazF cleaves cellular mRNAs specifically at ACA to block protein synthesis in Escherichia coli》**
- **作者**: Zhang Y., Zhang J., Hoeflich K.P., et al.
- **摘要**: 研究证实重组mazF蛋白特异性识别并切割mRNA中的ACA序列，导致翻译终止。实验通过体外mRNA降解分析和体内生长抑制实验验证了其底物选择性和生物学功能。
4. **《Controlled expression of the toxin gene mazF in Bacillus subtilis》**
- **作者**: Nariya H., Inouye M.
- **摘要**: 在枯草芽孢杆菌中构建了诱导型重组mazF表达系统，发现mazF的诱导表达可显著抑制细菌生长并引发细胞凋亡，为研究毒素-抗毒素系统在合成生物学中的应用提供了新策略。
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以上文献涵盖了mazF重组蛋白的**功能机制**、**结构解析**、**底物特异性**及**生物技术应用**方向的研究。
MazF is a sequence-specific endoribonuclease that functions as part of the bacterial toxin-antitoxin (TA) system *mazEF*, first identified in *Escherichia coli*. TA systems are genetic modules where a stable toxin protein (e.g., MazF) is co-expressed with a labile antitoxin (MazE) to regulate bacterial survival under stress. Under normal conditions, MazE binds and neutralizes MazF. However, under nutrient deprivation, DNA damage, or antibiotic exposure, MazE is degraded, freeing MazF to cleave single-stranded RNA at specific sequences (e.g., ACA motifs), halting translation and inducing growth arrest or programmed cell death. This mechanism is proposed to promote bacterial persistence, biofilm formation, or altruistic population behavior during stress.
As a recombinant protein, MazF is engineered for heterologous expression in various hosts (e.g., *E. coli*, mammalian cells) to study its biochemical properties or exploit its RNA-cleaving activity. Researchers utilize recombinant MazF to dissect RNA metabolism, develop RNA-based tools, or engineer synthetic gene circuits. Its sequence specificity enables applications in mRNA mapping, transcriptome analysis, or controlled protein synthesis in synthetic biology.
Recent studies explore MazF’s potential in biotechnology and medicine, such as targeting persister cells in infections or serving as a controllable "kill switch" in genetically modified organisms. However, its role in bacterial stress adaptation remains debated, with ongoing research addressing its physiological relevance and evolutionary conservation across prokaryotes. Recombinant MazF continues to be a valuable tool for understanding post-transcriptional regulation and developing RNA-targeted technologies.
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