| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CATSPER1 |
| Uniprot No | Q8NEC5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-445aa |
| 氨基酸序列 | MDQNSVPEKAQNEADTNNADRFFRSHSSPPHHRPGHSRALHHYELHHHGVPHQRGESHHPPEFQDFHDQALSSHVHQSHHHSEARNHGRAHGPTGFGLAPSQGAVPSHRSYGEDYHDELQRDGRRHHDGSQYGGFHQQSDSHYHRGSHHGRPQYLGENLSHYSSGVPHHGEASHHGGSYLPHGPNPYSESFHHSEASHLSGLQHDESQHHQVPHRGWPHHHQVHHHGRSRHHEAHQHGKSPHHGETISPHSSVGSYQRGISDYHSEYHQGDHHPSEYHHGDHPHHTQHHYHQTHRHRDYHQHQDHHGAYHSSYLHGDYVQSTSQLSIPHTSRSLIHDAPGPAASRTGVFPYHVAHPRGSAHSMTRSSSTIRSRVTQMSKKVHTQDISTKHSEDWGKEEGQFQKRKTGRLQRTRKKGHSTNLFQWLWEKLTFLIQGFREMIRNLTQ |
| 预测分子量 | 52.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CATSPER1重组蛋白的3篇参考文献示例(部分信息为示例性概括)：
1. **文献名称**: "Functional characterization of recombinant human CATSPER1 channel in sperm motility"
**作者**: Lobley A, et al.
**摘要**: 该研究通过重组表达人源CATSPER1蛋白，证实其在精子钙离子内流和运动调控中的关键作用，揭示其与男性生育能力的直接关联。
2. **文献名称**: "Structural insights into the CATSPER1 complex using cryo-EM"
**作者**: Chung JJ, et al.
**摘要**: 利用冷冻电镜解析CATSPER1蛋白复合体的三维结构，阐明其作为钙离子通道的分子机制，为不育症相关突变研究提供结构基础。
3. **文献名称**: "Expression and purification of recombinant CATSPER1 for antibody development"
**作者**: Smith AB, et al.
**摘要**: 报道了在HEK293细胞中高效表达并纯化重组CATSPER1蛋白的方法，成功制备特异性抗体，用于男性不育的分子诊断工具开发。
4. **文献名称**: "CATSPER1 channel modulators: High-throughput screening using recombinant protein"
**作者**: Zeng X, et al.
**摘要**: 基于重组CATSPER1蛋白建立药物筛选平台，发现新型小分子激动剂/抑制剂，为避孕或生育治疗药物研发提供潜在靶点。
(注：上述文献为示例，实际引用需根据具体研究核实原文信息。)
**Background of CATSPER1 Recombinant Protein**
CATSPER1 is a key subunit of the CATSPER channel complex, a sperm-specific, pH-dependent calcium ion channel critical for sperm hyperactivation, motility, and fertilization. This transmembrane protein is exclusively expressed in the principal piece of the sperm flagellum and plays a pivotal role in regulating calcium influx during sperm capacitation. Mutations in the *CATSPER1* gene are linked to male infertility due to impaired sperm function.
Recombinant CATSPER1 protein is engineered to study the structure, function, and regulation of the CATSPER channel. Produced via heterologous expression systems (e.g., HEK293 cells or insect cells), it often includes affinity tags (e.g., His-tag) for purification. Its applications span *in vitro* studies to dissect ion selectivity, channel gating mechanisms, and interactions with auxiliary subunits (CATSPERβ, γ, δ) or regulatory proteins.
Research using CATSPER1 recombinant protein has advanced understanding of calcium signaling defects in infertile males. It also aids in drug discovery, enabling high-throughput screening of small molecules to modulate channel activity—potential therapeutics for infertility or non-hormonal contraceptives. Structural studies (cryo-EM, crystallography) leveraging recombinant CATSPER1 have revealed insights into channel architecture and activation by alkalization.
Despite progress, challenges remain, including the channel’s complex heteromeric assembly and instability *in vitro*. Current efforts focus on optimizing recombinant expression to reconstitute full-channel functionality, facilitating mechanistic and translational studies. CATSPER1 recombinant proteins thus serve as vital tools bridging basic reproductive biology and clinical applications.
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