| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASP2 |
| Uniprot No | P56817 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-501aa |
| 氨基酸序列 | MAQALPWLLLWMGAGVLPAHGTQHGIRLPLRSGLGGAPLGLRLPRETDEEPEEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGAGFPLNQSEVLASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAISQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTLDMEDCGYNIPQTDESTLMTIAYVMAAICALFMLPLCLMVCQWRCLRCLRQQHDDFADDISLLK |
| 预测分子量 | 55,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ASP2(假设为BACE2)重组蛋白研究的模拟参考文献示例，供参考：
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1. **标题**: "Expression and Purification of Recombinant Human BACE2 in Escherichia coli"
**作者**: Zhang L, et al.
**摘要**: 研究报道了利用大肠杆菌系统高效表达人源BACE2重组蛋白，通过亲和层析和凝胶过滤纯化，获得高纯度蛋白。酶活性分析表明重组BACE2具有底物特异性，为后续药物筛选提供材料。
2. **标题**: "Structural Insights into BACE2 by X-ray Crystallography: Implications for Substrate Specificity"
**作者**: Patel S, et al.
**摘要**: 通过X射线晶体学解析BACE2重组蛋白的3D结构，揭示其与BACE1的活性位点差异，解释了二者在淀粉样蛋白加工中的不同作用，为选择性抑制剂设计奠定基础。
3. **标题**: "Functional Characterization of Recombinant BACE2 in Cellular Models of Diabetes"
**作者**: Kim H, et al.
**摘要**: 在哺乳动物细胞中表达重组BACE2.发现其在胰岛β细胞中调控胰岛素受体加工，体外实验证实其酶活性受pH和离子强度影响，提示BACE2在糖尿病病理中的潜在作用。
4. **标题**: "Optimization of Eukaryotic Expression Systems for Enhanced BACE2 Production"
**作者**: Müller R, et al.
**摘要**: 对比昆虫细胞和哺乳动物系统表达BACE2的效率，优化分泌信号肽和培养条件，显著提高蛋白产量及稳定性，适用于大规模功能研究和抗体开发。
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**注意**：以上为模拟生成的示例文献，实际文献需通过PubMed、Google Scholar等平台检索关键词(如“BACE2 recombinant”、“ASP2 protein expression”)获取。建议结合具体研究方向筛选近年高被引论文。
**Background of ASP2 Recombinant Protein**
ASP2 (Amyloid Structural Protein 2) recombinant protein is a genetically engineered biomolecule derived from the ASP2 gene, which encodes a protein implicated in amyloidogenesis and neurodegenerative disorders. ASP2 is structurally characterized by its β-sheet-rich domains, which contribute to its propensity to aggregate into amyloid fibrils—a hallmark of diseases like Alzheimer’s. The native protein is involved in cellular processes such as lipid metabolism, synaptic function, and intracellular signaling, though its exact physiological role remains under investigation.
Recombinant ASP2 is produced using expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and proper folding. Its production enables controlled studies on amyloid formation mechanisms, toxicity, and interactions with other biomolecules. Researchers utilize ASP2 recombinant protein to model pathological aggregation *in vitro*, screen potential inhibitors of amyloid fibrillization, and explore therapeutic strategies targeting amyloid-related diseases.
Additionally, ASP2’s recombinant form has applications in structural biology, aiding in X-ray crystallography or cryo-EM studies to resolve its 3D conformation and aggregation pathways. Recent studies also highlight its potential as a biomarker or immunogen for diagnostic or vaccine development. Challenges persist in mimicking post-translational modifications *in vitro* and maintaining solubility during experiments, driving innovations in protein engineering.
Overall, ASP2 recombinant protein serves as a critical tool for unraveling amyloid pathology and advancing therapeutic interventions for neurodegenerative conditions. Its study bridges molecular insights with translational applications, addressing unmet needs in neurology and drug discovery.
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