| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | BAR1 |
| Uniprot No | P12630 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 43-395aa |
| 氨基酸序列 | MYYATTLDIGTPSQSLTVLFDTGSADFWVMDSSNPFCLPNSNTSSYSNATYNGEEVKPSIDCRSMSTYNEHRSSTYQYLENGRFYITYADGTFADGSWGTETVSINGIDIPNIQFGVAKYATTPVSGVLGIGFPRRESVKGYEGAPNEYYPNFPQILKSEKIIDVVAYSLFLNSPDSGTGSIVFGAIDESKFSGDLFTFPMVNEYPTIVDAPATLAMTIQGLGAQNKSSCEHETFTTTKYPVLLDSGTSLLNAPKVIADKMASFVNASYSEEEGIYILDCPVSVGDVEYNFDFGDLQISVPLSSLILSPETEGSYCGFAVQPTNDSMVLGDVFLSSAYVVFDLDNYKISLAQA |
| 预测分子量 | 44.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BAR1重组蛋白的3篇参考文献示例，涵盖不同研究重点：
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1. **文献名称**：*"Purification and Functional Analysis of Recombinant BAR1 Protease in Saccharomyces cerevisiae"*
**作者**：Smith, J. et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组BAR1蛋白酶，证实其可特异性水解α因子交配信息素，揭示了BAR1通过调控信息素浓度维持酵母有性生殖动态平衡的分子机制。
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2. **文献名称**：*"Structural Insights into the BAR1-α Factor Interaction by Cryo-EM"*
**作者**：Chen, L. & Wang, H.
**摘要**：通过冷冻电镜解析重组BAR1蛋白与α因子的复合物结构，揭示了BAR1底物结合位点的关键氨基酸残基，为设计靶向BAR1的酶活性抑制剂提供了结构基础。
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3. **文献名称**：*"Heterologous Expression of BAR1 in Pichia pastoris Enhances Pheromone Degradation Efficiency"*
**作者**：Rodriguez, M. et al.
**摘要**：在毕赤酵母系统中高效表达重组BAR1蛋白，优化发酵条件后酶活性较天然酵母提升5倍，该工程化蛋白在工业微生物共培养体系中展现出调控群体感应的应用潜力。
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**注**：以上文献为示例性内容，实际研究中请根据具体需求检索权威数据库(如PubMed、Web of Science)获取真实文献。若需某类研究的扩展(如疾病关联或药物开发)，可进一步补充关键词筛选。
**Background of BAR1 Recombinant Protein**
The BAR1 recombinant protein is derived from the *BAR1* gene, which encodes a member of the **BAR (Bin-Amphiphysin-Rvs) domain-containing protein family**. These proteins are characterized by their unique crescent-shaped BAR domains, enabling them to sense and induce membrane curvature, a critical function in membrane remodeling processes such as vesicle formation, endocytosis, and intracellular trafficking. BAR1 is particularly notable for its role in regulating cellular dynamics and signaling pathways.
In molecular biology, recombinant BAR1 is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its structural and functional properties. Its production typically involves cloning the *BAR1* gene into expression vectors, followed by purification using affinity tags (e.g., His-tag). The recombinant protein retains the ability to bind lipid membranes and interact with partner proteins, making it a valuable tool for *in vitro* assays, including liposome binding studies, cytoskeleton interaction analyses, and structural biology (e.g., crystallography or cryo-EM).
BAR1 has been implicated in diseases such as cancer and neurodegenerative disorders, where membrane dysregulation occurs. Researchers leverage recombinant BAR1 to dissect its role in pathological mechanisms and screen for therapeutic compounds targeting membrane-associated processes. Additionally, its study contributes to understanding fundamental cell biology, such as how cells maintain membrane integrity during division or migration. Overall, BAR1 recombinant protein serves as a cornerstone for exploring membrane dynamics and developing biomedical applications.
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