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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IFNB1 |
| Uniprot No | P01574 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-187aa |
| 氨基酸序列 | MSYNLLGFLQRSSNFQCQKLLWQLNGRLEYCLKDRMNFDIPEEIKQLQQFQKEDAALTIYEMLQNIFAIFRQDSSSTGWNETIVENLLANVYHQINHLKTVLEEKLEKEDFTRGKLMSSLHLKRYYGRILHYLKAKEYSHCAWTIVRVEILRNFYFINRLTGYLRN |
| 预测分子量 | 20.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IFNB1(干扰素β-1)重组蛋白的3篇代表性文献信息：
1. **文献名称**：Recombinant interferon-beta for the treatment of multiple sclerosis: a meta-analysis
**作者**：Rudick RA, et al.
**摘要**：该研究通过荟萃分析评估重组干扰素β-1在治疗多发性硬化症(MS)中的疗效，发现其显著降低复发率和MRI病灶活动，支持其作为MS的一线治疗药物。
2. **文献名称**：Production and characterization of bioactive recombinant human interferon-beta in Escherichia coli
**作者**：Karpusas M, et al.
**摘要**：研究报道了利用大肠杆菌表达系统高效生产重组人干扰素β-1的方法，并通过结构分析和体外实验验证了其抗病毒活性及与受体的结合能力。
3. **文献名称**：Structural basis of the differential activation of interferon-beta gene by NF-κB p50 homodimer
**作者**：Sasaki N, et al.
**摘要**：该文献解析了NF-κB p50同源二聚体调控IFNB1基因表达的分子机制，揭示了重组干扰素β在先天免疫反应中的转录调控网络。
注：以上文献为示例，实际引用时建议通过PubMed或Web of Science核对最新研究。
Interferon beta-1 (IFN-β1), a member of the type I interferon family, is a naturally occurring cytokine with antiviral, antiproliferative, and immunomodulatory properties. Discovered in the late 1970s, it plays a critical role in the innate immune response by binding to cell surface receptors (IFNAR1/2) and activating the JAK-STAT signaling pathway, which induces the expression of interferon-stimulated genes (ISGs). These genes orchestrate antiviral defenses, modulate immune cell activity, and inhibit viral replication.
Recombinant IFN-β1. produced via genetic engineering in mammalian cell systems (e.g., Chinese Hamster Ovary cells), mimics the human protein but is optimized for therapeutic use. Two major isoforms, IFN-β1a (glycosylated, identical to natural IFN-β) and IFN-β1b (non-glycosylated, serine-substituted), are clinically used. Approved in the 1990s, recombinant IFN-β1 became a cornerstone therapy for relapsing-remitting multiple sclerosis (MS), reducing relapse rates by suppressing autoreactive T-cells, limiting blood-brain barrier disruption, and promoting regulatory immune responses.
Its production ensures high purity and batch consistency, addressing limitations of early interferon therapies derived from human cells. Beyond MS, IFN-β1 has shown potential in treating viral infections (e.g., hepatitis, COVID-19) and certain cancers, though side effects like flu-like symptoms and neutralizing antibody development remain challenges. Ongoing research explores pegylated formulations and combination therapies to enhance efficacy and patient tolerance. As a benchmark biologic, IFN-β1 exemplifies the integration of molecular biology and immunology in developing targeted therapeutics.
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