纯度 | >90%SDS-PAGE. |
种属 | E.coli |
靶点 | PGRP-SC2 |
Uniprot No | Q9V4X2 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 21-184aa |
氨基酸序列 | VTIISKSEWGGRSATSKTSLANYLSYAVIHHTAGNYCSTKAACITQLQNIQAYHMDSLGWADIGYNFLIGGDGNVYEGRGWNVMGAHATNWNSKSIGISFLGNYNTNTLTSAQITAAKGLLSDAVSRGQIVSGYILYGHRQVGSTECPGTNIWNEIRTWSNWKA |
预测分子量 | 25.2 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PGRP-SC2重组蛋白的3篇参考文献示例(内容基于公开研究整理,非真实文献):
1. **《Peptidoglycan recognition protein SC2 regulates gut microbiota homeostasis in Drosophila》**
- **作者**: Li H, et al.
- **摘要**: 研究通过重组PGRP-SC2蛋白发现其具有酰胺酶活性,可降解肠道共生菌的肽聚糖,维持果蝇肠道免疫稳态,并防止过度免疫反应对宿主的损伤。
2. **《Structural and functional analysis of recombinant PGRP-SC2 in immune pathway modulation》**
- **作者**: Zhang Y, et al.
- **摘要**: 解析了重组PGRP-SC2蛋白的晶体结构,证明其通过结合革兰氏阴性菌肽聚糖,抑制Imd信号通路过度激活,平衡果蝇对抗病原体与免疫耐受的关系。
3. **《Recombinant PGRP-SC2 suppresses septic shock in a murine model》**
- **作者**: Wang X, et al.
- **摘要**: 利用原核表达系统制备重组PGRP-SC2.在小鼠脓毒症模型中验证其通过中和循环中过量肽聚糖,降低促炎因子水平,缓解全身性炎症反应。
注:以上文献名为示例,实际研究中建议通过PubMed或Web of Science以关键词“PGRP-SC2 recombinant”检索最新论文。
**Background of PGRP-SC2 Recombinant Protein**
Peptidoglycan Recognition Protein-SC2 (PGRP-SC2) belongs to the PGRP family, a group of evolutionarily conserved pattern recognition receptors critical in innate immunity. These proteins detect peptidoglycan (PGN), a key component of bacterial cell walls, and initiate immune responses. PGRP-SC2. primarily studied in *Drosophila melanogaster*, is a secreted protein with dual functionality: it acts as a sensor for microbial invasion and a regulator of immune homeostasis. Unlike other PGRPs that trigger antimicrobial pathways, PGRP-SC2 is involved in modulating excessive immune activation, preventing detrimental inflammation.
Structurally, PGRP-SC2 contains a conserved PGRP domain that binds to PGN fragments. However, it lacks enzymatic activity for PGN degradation, distinguishing it from catalytic PGRPs (e.g., PGRP-LB). Instead, it sequesters immunogenic PGN fragments, thereby inhibiting overactivation of the IMD (Immune Deficiency) pathway, a signaling cascade responsible for antibacterial responses in insects. This regulatory role is vital for maintaining immune balance, particularly during chronic infections or when commensal microbes are present.
Recombinant PGRP-SC2 is produced via genetic engineering, often expressed in systems like *E. coli* or insect cell cultures, enabling studies on its structure, ligand interactions, and immunomodulatory mechanisms. Research highlights its potential in understanding immune tolerance, host-microbe interactions, and inflammatory diseases. For instance, dysregulation of PGRP-SC2 homologs in mammals has been linked to autoimmune disorders, suggesting translational relevance. Its recombinant form also serves as a tool for developing anti-inflammatory therapies or enhancing microbial resistance in agricultural applications.
In summary, PGRP-SC2 exemplifies a critical immune checkpoint protein, balancing pathogen defense and tissue integrity, with recombinant versions facilitating mechanistic and applied research across immunology and biotechnology.
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