Recombinant E.coli PGRP-SC2 protein

中文名： 肽聚糖识别蛋白SC2(PGRP-SC2)重组蛋白
别名： PGRP-SC2；Peptidoglycan-recognition protein SC2

货号: PA2000-4548

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	E.coli
靶点	PGRP-SC2
Uniprot No	Q9V4X2
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	21-184aa
氨基酸序列	VTIISKSEWGGRSATSKTSLANYLSYAVIHHTAGNYCSTKAACITQLQNIQAYHMDSLGWADIGYNFLIGGDGNVYEGRGWNVMGAHATNWNSKSIGISFLGNYNTNTLTSAQITAAKGLLSDAVSRGQIVSGYILYGHRQVGSTECPGTNIWNEIRTWSNWKA
预测分子量	25.2 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于PGRP-SC2重组蛋白的3篇参考文献示例(内容基于公开研究整理，非真实文献)：

1. **《Peptidoglycan recognition protein SC2 regulates gut microbiota homeostasis in Drosophila》**

- **作者**: Li H, et al.

- **摘要**: 研究通过重组PGRP-SC2蛋白发现其具有酰胺酶活性，可降解肠道共生菌的肽聚糖，维持果蝇肠道免疫稳态，并防止过度免疫反应对宿主的损伤。

2. **《Structural and functional analysis of recombinant PGRP-SC2 in immune pathway modulation》**

- **作者**: Zhang Y, et al.

- **摘要**: 解析了重组PGRP-SC2蛋白的晶体结构，证明其通过结合革兰氏阴性菌肽聚糖，抑制Imd信号通路过度激活，平衡果蝇对抗病原体与免疫耐受的关系。

3. **《Recombinant PGRP-SC2 suppresses septic shock in a murine model》**

- **作者**: Wang X, et al.

- **摘要**: 利用原核表达系统制备重组PGRP-SC2.在小鼠脓毒症模型中验证其通过中和循环中过量肽聚糖，降低促炎因子水平，缓解全身性炎症反应。

注：以上文献名为示例，实际研究中建议通过PubMed或Web of Science以关键词“PGRP-SC2 recombinant”检索最新论文。

背景信息

**Background of PGRP-SC2 Recombinant Protein**

Peptidoglycan Recognition Protein-SC2 (PGRP-SC2) belongs to the PGRP family, a group of evolutionarily conserved pattern recognition receptors critical in innate immunity. These proteins detect peptidoglycan (PGN), a key component of bacterial cell walls, and initiate immune responses. PGRP-SC2. primarily studied in *Drosophila melanogaster*, is a secreted protein with dual functionality: it acts as a sensor for microbial invasion and a regulator of immune homeostasis. Unlike other PGRPs that trigger antimicrobial pathways, PGRP-SC2 is involved in modulating excessive immune activation, preventing detrimental inflammation.

Structurally, PGRP-SC2 contains a conserved PGRP domain that binds to PGN fragments. However, it lacks enzymatic activity for PGN degradation, distinguishing it from catalytic PGRPs (e.g., PGRP-LB). Instead, it sequesters immunogenic PGN fragments, thereby inhibiting overactivation of the IMD (Immune Deficiency) pathway, a signaling cascade responsible for antibacterial responses in insects. This regulatory role is vital for maintaining immune balance, particularly during chronic infections or when commensal microbes are present.

Recombinant PGRP-SC2 is produced via genetic engineering, often expressed in systems like *E. coli* or insect cell cultures, enabling studies on its structure, ligand interactions, and immunomodulatory mechanisms. Research highlights its potential in understanding immune tolerance, host-microbe interactions, and inflammatory diseases. For instance, dysregulation of PGRP-SC2 homologs in mammals has been linked to autoimmune disorders, suggesting translational relevance. Its recombinant form also serves as a tool for developing anti-inflammatory therapies or enhancing microbial resistance in agricultural applications.

In summary, PGRP-SC2 exemplifies a critical immune checkpoint protein, balancing pathogen defense and tissue integrity, with recombinant versions facilitating mechanistic and applied research across immunology and biotechnology.