| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | splD |
| Uniprot No | Q2FXC5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 37-239aa |
| 氨基酸序列 | ENSVKLITNTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYKVTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPNGNKLQMYESTGKVLSVNGNIVTSDAVVQPGSSGSPILNSKREAIGVMYASDKPTGESTRSFAVYFSPEIKKFIADNLDK |
| 预测分子量 | 29.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于splD重组蛋白的3篇参考文献概述(注：文献为模拟示例，实际研究需通过学术数据库检索确认)：
1. **文献名称**：*Recombinant SplD protease from Staphylococcus aureus: purification and characterization*
**作者**：Smith A, et al.
**摘要**：本研究成功克隆并表达了金黄色葡萄球菌splD基因的重组蛋白，通过亲和层析纯化后分析了其蛋白酶活性，发现SplD在特定pH下对宿主细胞外基质蛋白具有降解作用。
2. **文献名称**：*Role of SplD in Staphylococcus aureus virulence: Insights from a recombinant protein model*
**作者**：Chen L, et al.
**摘要**：利用重组SplD蛋白进行小鼠感染模型实验，证明其通过抑制中性粒细胞趋化性加剧感染，揭示了splD在金黄色葡萄球菌免疫逃逸中的潜在机制。
3. **文献名称**：*Structural and functional analysis of the staphylococcal SplD protease*
**作者**：Tanaka K, et al.
**摘要**：通过X射线晶体学解析了重组SplD蛋白的三维结构，结合突变体实验验证了其催化活性中心的保守氨基酸残基，为开发特异性抑制剂提供了结构基础。
**提示**：实际研究中splD相关文献较少，建议拓展检索"spl蛋白酶家族"或"金黄色葡萄球菌胞外蛋白酶"。若需真实文献，可尝试在PubMed中以“SplD AND (recombinant OR expression)”为关键词检索。
**Background of splD Recombinant Protein**
splD is a serine protease-like protein (Spl) encoded by the *splD* gene, part of the Spl operon (*splA-F*) in *Staphylococcus aureus*, a Gram-positive pathogen associated with diverse infections. The Spl family shares structural homology with serine proteases but exhibits limited or atypical proteolytic activity. splD, like other Spl proteins, is speculated to play a role in immune evasion or host-pathogen interactions, though its exact biological function remains unclear. Studies suggest Spl proteins may degrade host defense molecules (e.g., antimicrobial peptides) or modulate immune responses, contributing to bacterial virulence.
Recombinant splD protein is produced via genetic engineering, typically by cloning the *splD* gene into expression vectors (e.g., *E. coli*), followed by purification. This allows researchers to study its structure, enzymatic properties, and interactions with host proteins without the complexity of native bacterial systems. Recombinant splD has been utilized to investigate its potential role in *S. aureus* pathogenesis, including adhesion, biofilm formation, or resistance to innate immunity.
Interest in splD also stems from its potential as a therapeutic target or diagnostic marker. Inhibitors targeting Spl proteins could offer novel anti-infective strategies, while antibodies against splD might aid in detecting *S. aureus* infections. However, challenges persist in clarifying its precise mechanism and validating its clinical relevance.
Overall, splD recombinant protein serves as a critical tool for unraveling the pathogenic strategies of *S. aureus* and exploring applications in antimicrobial development. Further research is needed to bridge gaps in understanding its contribution to bacterial fitness and disease progression.
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