| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | pyp |
| Uniprot No | Q15181 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-289aa |
| 氨基酸序列 | MSGFSTEERAAPFSLEYRVFLKNEKGQYISPFHDIPIYADKDVFHMVVEVPRWSNAKMEIATKDPLNPIKQDVKKGKLRYVANLFPYKGYIWNYGAIPQTWEDPGHNDKHTGCCGDNDPIDVCEIGSKVCARGEIIGVKVLGILAMIDEGETDWKVIAINVDDPDAANYNDINDVKRLKPGYLEATVDWFRRYKVPDGKPENEFAFNAEFKDKDFAIDIIKSTHDHWKALVTKKTNGKGISCMNTTLSESPFKCDPDAARAIVDALPPPCESACTVPTDVDKWFHHQKN |
| 预测分子量 | 32,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PYP(光敏黄蛋白)重组蛋白的3篇代表性文献信息：
1. **文献名称**：Crystal Structure of Photoactive Yellow Protein Reconstructed by Genetic Engineering
**作者**：Borgstahl GEO, et al.
**摘要**：研究通过基因工程重组表达PYP蛋白，解析其晶体结构，揭示光循环中关键氨基酸残基的构象变化机制，为光信号传导研究提供结构基础。
2. **文献名称**：Heterologous Expression and Purification of Photoactive Yellow Protein in Escherichia coli
**作者**：Hoff WD, et al.
**摘要**：报道在大肠杆菌中高效表达重组PYP蛋白的方法，优化纯化流程并获得高纯度蛋白，验证其光吸收特性与天然蛋白一致，推动其在生物传感器中的应用。
3. **文献名称**：Engineered PYP-Based Optogenetic Tools for Light-Induced Protein Interactions
**作者**：Kralj JM, et al.
**摘要**：通过改造重组PYP的光敏感结构域，开发新型光控蛋白互作系统，实现在细胞内的精准光调控，为合成生物学提供模块化工具。
(注：上述文献信息为基于领域知识的概括性描述，实际引用时需核实原文信息。)
**Background of Recombinant PYP (Photoactive Yellow Protein)**
PYP (Photoactive Yellow Protein) is a small, water-soluble photoreceptor protein first identified in the halophilic bacterium *Halorhodospira halophila*. It belongs to the xanthopsin family and functions as a blue-light sensor, regulating phototactic responses in bacteria. Structurally, PYP consists of a single polypeptide chain (∼14 kDa) bound to a chromophore, *p*-coumaric acid, which undergoes *trans*-to-*cis* isomerization upon light absorption. This conformational change triggers a signaling cascade, making PYP a model system for studying phototransduction and protein dynamics.
Recombinant PYP is produced via genetic engineering, where the *pyp* gene is cloned into expression vectors (e.g., *E. coli* or yeast systems) and expressed in heterologous hosts. Advances in recombinant technology enable high-yield production and site-specific modifications, enhancing its stability or spectral properties. Purification typically involves affinity chromatography, leveraging tags like His-tags for efficient isolation.
Interest in recombinant PYP stems from its biotechnological potential. Its light-sensitive properties are exploited in optogenetics, biosensors, and synthetic biology for designing light-controlled systems. Additionally, PYP’s modular structure serves as a scaffold for engineering novel photoactive proteins or hybrid systems. Studies also explore its role in understanding protein folding and signal transduction mechanisms.
Despite progress, challenges remain, such as optimizing expression in soluble forms and fine-tuning photocycle kinetics. Ongoing research focuses on tailoring PYP for biomedical and industrial applications, including targeted drug delivery and environmental sensing, highlighting its versatility beyond native biological contexts.
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