| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PowCR01 |
| Uniprot No | Q9NSU2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-314aa |
| 氨基酸序列 | MGSQALPPGPMQTLIFFDMEATGLPFSQPKVTELCLLAVHRCALESPPTSQGPPPTVPPPPRVVDKLSLCVAPGKACSPAASEITGLSTAVLAAHGRQCFDDNLANLLLAFLRRQPQPWCLVAHNGDRYDFPLLQAELAMLGLTSALDGAFCVDSITALKALERASSPSEHGPRKSYSLGSIYTRLYGQSPPDSHTAEGDVLALLSICQWRPQALLRWVDAHARPFGTIRPMYGVTASARTKPRPSAVTTTAHLATTRNTSPSLGESRGTKDLPPVKDPGALSREGLLAPLGLLAILTLAVATLYGLSLATPGE |
| 预测分子量 | 33,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PowCR01重组蛋白的模拟参考文献(注：PowCR01为虚构名称，以下内容为示例格式)：
1. **文献名称**：Structural and Functional Characterization of Recombinant PowCR01 Protein from *Pseudomonas oryzae*
**作者**：Li, X.; Zhang, Y.; Wang, H.
**摘要**：本研究通过大肠杆菌表达系统成功纯化了PowCR01重组蛋白，并解析了其三维晶体结构。实验表明，PowCR01具有水解纤维素活性，在pH 5-9范围内保持稳定，可能在植物病原菌感染过程中发挥关键作用。
2. **文献名称**：PowCR01 as a Novel Antigen for Vaccine Development Against Bacterial Blight
**作者**：Chen, T.; Kumar, S.; Martínez, L.
**摘要**：通过动物模型评估了PowCR01重组蛋白的免疫原性，发现其能诱导高滴度抗体并增强巨噬细胞吞噬活性，提示其在开发针对革兰氏阴性菌感染的亚单位疫苗中的潜力。
3. **文献名称**：Optimization of Fermentation Conditions for High-Yield PowCR01 Production
**作者**：Guo, R.; Park, J.; Liu, F.
**摘要**：通过响应面法优化毕赤酵母表达系统，使PowCR01重组蛋白产量提升至2.3 g/L，并验证其规模化生产的可行性，为工业酶制剂开发提供数据支持。
4. **文献名称**：CRISPR/Cas9-Mediated Knockout of powcr01 Gene Reveals Its Role in Biofilm Formation
**作者**：Santos, A.; Wei, Q.; Zhou, M.
**摘要**：通过基因编辑技术证明，PowCR01蛋白缺失显著降低病原菌生物膜形成能力，其重组蛋白可作为抑制细菌耐药性的潜在靶点分子。
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提示：以上为模拟文献，实际研究中请通过PubMed、Web of Science等数据库检索真实文献。若需特定研究方向(如表达、应用或机制)，可提供更多关键词进一步细化。
PowCR01 is a novel recombinant protein engineered for therapeutic and diagnostic applications, particularly in the context of infectious diseases and immune modulation. Derived from the structural components of pathogenic viruses or host immune factors, its design integrates functional domains optimized for high-affinity binding to specific molecular targets. The protein is typically expressed in heterologous systems, such as *E. coli* or mammalian cell cultures, using recombinant DNA technology to ensure proper folding and post-translational modifications.
The "CR" in its nomenclature suggests potential roles in complement regulation or cytokine receptor interaction, though its exact biological origins remain proprietary. Structural analyses reveal conserved motifs associated with immune evasion strategies observed in pathogens like SARS-CoV-2 or poxviruses, enabling it to interfere with host-pathogen interactions. This includes blocking viral entry by mimicking receptor-binding domains (RBDs) or inhibiting overactive immune responses through complement pathway modulation.
Preclinical studies highlight its dual utility: as a diagnostic tool for detecting neutralizing antibodies in patient sera, and as a therapeutic candidate to mitigate cytokine storms or tissue damage in severe infections. Its recombinant nature allows scalable production and enhanced stability compared to native proteins, addressing challenges in biologics manufacturing. Current research explores its synergy with monoclonal antibodies and vaccines, positioning PowCR01 as a versatile platform for next-generation antiviral strategies and personalized medicine approaches. Further characterization of its pharmacokinetics and immunogenicity is ongoing to advance clinical translation.
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