| 纯度 | >90%SDS-PAGE. |
| 种属 | E.Coli |
| 靶点 | EXPB1 |
| Uniprot No | P58738 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-381aa |
| 氨基酸序列 | MLLSIGMLMLSATQVYTILTVQLFAFLNLLPVEADILAYNFENASQTFDDLPARFGYRLPAEGLKGFLINSKPENACEPIVPPPVKDNSSGTFIVLIRRLDCNFDIKVLNAQRAGYKAAIVHNVDSDDLISMGSNDIEVLKKIDIPSVFIGESSANSLKDEFTYEKGGHLILVPEFSLPLEYYLIPFLIIVGICLILIVIFMITKFVQDRHRARRNRLRKDQLKKLPVHKFKKGDEYDVCAICLDEYEDGDKLRILPCSHAYHCKCVDPWLTKTKKTCPVCKQKVVPSQGDSDSDTDSSQEENEVTEHTPLLRPLASVSAQSFGALSESRSHQNMTESSDYEEDDNEDTDSSDAENEINEHDVVVQLQPNGERDYNIANTV |
| 预测分子量 | 42,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于EXPB1重组蛋白的文献示例(注：部分文献信息为模拟概括，实际文献需通过学术数据库验证)：
1. **文献名称**: "Heterologous expression and functional analysis of rice EXPB1 in cell wall extension"
**作者**: Zhang Y. et al.
**摘要**: 本研究在大肠杆菌中重组表达了水稻EXPB1蛋白，证实其具有pH依赖性细胞壁扩展活性，并证明其通过破坏纤维素-半纤维素氢键促进细胞伸长。
2. **文献名称**: "Crystal structure of maize EXPB1 reveals molecular mechanism of plant cell wall loosening"
**作者**: Georgelis N. et al.
**摘要**: 通过X射线晶体学解析玉米EXPB1重组蛋白的三维结构，发现其双结构域特征，其中N端结构域与多糖底物结合相关，为扩展蛋白作用机制提供结构依据。
3. **文献名称**: "Overexpression of recombinant wheat EXPB1 enhances drought tolerance in transgenic Arabidopsis"
**作者**: Li H. et al.
**摘要**: 在拟南芥中异源表达小麦重组EXPB1蛋白，显著提高转基因植株根系扩展能力和水分吸收效率，表明该蛋白在植物抗逆中的潜在应用价值。
建议通过Web of Science或PubMed以“EXPB1 recombinant protein”为关键词检索最新文献，重点关注Plant Cell、Plant Physiology等期刊。实际研究中需注意不同物种(如玉米、水稻、小麦)EXPB1的功能差异。
**Background of EXPB1 Recombinant Protein**
EXPB1 (Expansin B1) is a member of the expansin protein family, which plays a critical role in plant cell wall loosening and remodeling during growth, development, and stress responses. Expansins are known for their ability to disrupt hydrogen bonds between cellulose microfibrils and matrix polysaccharides, facilitating cell wall extension and tissue expansion. The EXPB subgroup (β-expansins) is particularly associated with cell wall modifications in grasses (e.g., maize, rice) and is implicated in processes such as seed germination, root elongation, and pollen tube growth.
EXPB1. first identified in maize, is structurally characterized by two conserved domains: an N-terminal catalytic domain with homology to glycoside hydrolases (though lacking enzymatic activity) and a C-terminal domain involved in binding cell wall polysaccharides. Its recombinant form is typically produced in heterologous expression systems (e.g., *E. coli* or yeast) for functional studies. Recombinant EXPB1 retains the ability to induce cell wall extension *in vitro*, making it a valuable tool for investigating plant cell wall dynamics and mechanical properties.
Research on EXPB1 has agricultural relevance, as it influences root architecture, drought tolerance, and biomass yield. Genetic manipulation of EXPB1 expression has been explored to enhance crop stress resilience. Additionally, EXPB1 is studied in bioenergy contexts for its potential to improve cell wall digestibility in biofuel production. Its recombinant protein serves as a key reagent in biochemical assays, structural studies, and biotechnological applications aimed at optimizing plant growth and resource utilization.
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