| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | H2-D1 |
| Uniprot No | P01900 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-311aa |
| 氨基酸序列 | GSHSLRYFVTAVSRPGFGEPRYMEVGYVDNTEFVRFDSDAENPRYEPRARWIEQEGPEYWERETRRAKGNEQSFRVDLRTALRYYNQSAGGSHTLQWMAGCDVESDGRLLRGYWQFAYDGCDYIALNEDLKTWTAADMAAQITRRKWEQAGAAERDRAYLEGECVEWLRRYLKNGNATLLRTDPPKAHVTHHRRPEGDVTLRCWALGFYPADITLTWQLNGEELTQEMELVETRPAGDGTFQKWASVVVPLGKEQKYTCHVEHEGLPEPLTLRWGKEEPPSSTKTNT |
| 预测分子量 | 39.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的关于H2-D1重组蛋白的参考文献示例(非真实文献，仅供格式参考)：
1. **文献名称**: "Expression and Purification of Recombinant H2-D1 for Structural Studies"
**作者**: Smith A, et al.
**摘要**: 本研究利用大肠杆菌表达系统成功表达了小鼠MHC-I分子H2-D1的重组蛋白，并通过亲和层析和尺寸排阻色谱实现了高纯度制备。结构分析表明该蛋白可正确折叠并与β2微球蛋白形成复合物，为后续抗原结合研究提供了基础。
2. **文献名称**: "H2-D1 Recombinant Protein in Antigen Presentation Assays"
**作者**: Johnson R, et al.
**摘要**: 通过昆虫细胞系统表达H2-D1重组蛋白，验证其与特定病毒肽段的结合能力。功能实验显示该重组蛋白可有效激活CD8+ T细胞，证明其在体外免疫应答研究中的实用性。
3. **文献名称**: "Crystal Structure of H2-D1 in Complex with a Tumor-Associated Peptide"
**作者**: Lee H, et al.
**摘要**: 解析了重组H2-D1蛋白与肿瘤抗原肽的复合物晶体结构(分辨率2.1Å)，揭示了其结合口袋的关键氨基酸残基，为设计基于MHC-I的肿瘤疫苗提供了结构依据。
4. **文献名称**: "Comparative Analysis of H2-D1 Recombinant Production in Prokaryotic and Eukaryotic Systems"
**作者**: Müller C, et al.
**摘要**: 对比了大肠杆菌、酵母和哺乳动物细胞表达H2-D1的效率和功能差异，发现哺乳动物系统表达的蛋白糖基化完整且稳定性最佳，但原核系统成本更低且适用于非糖基化研究。
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**提示**：以上为模拟示例，实际文献需通过PubMed/Google Scholar检索关键词(如"H2-D1 recombinant protein"+"MHC class I")。建议结合具体研究方向(结构生物学/免疫治疗等)筛选文献，并优先选择近5年高被引论文。
**Background of H2-D1 Recombinant Protein**
H2-D1 is a major histocompatibility complex (MHC) class I protein encoded by the *H2-D1* gene in mice, homologous to human HLA class I molecules. As a key component of the adaptive immune system, MHC class I proteins present antigenic peptides derived from intracellular pathogens or abnormal cellular proteins to CD8+ T cells, triggering immune responses. The H2-D1 molecule consists of a polymorphic heavy chain non-covalently associated with β2-microglobulin (β2m), forming a peptide-binding groove that accommodates 8–10 amino acid-long peptides.
Recombinant H2-D1 protein is typically produced in vitro using expression systems such as *E. coli* or mammalian cells (e.g., HEK293), often with tags (e.g., His-tag) for purification. Its production enables studies on peptide-MHC interactions, T cell receptor specificity, and immune evasion mechanisms. H2-D1 is widely used in murine models to investigate antiviral or antitumor immunity, autoimmune diseases, and transplantation biology. For example, researchers utilize H2-D1 tetramers loaded with specific peptides to track antigen-specific CD8+ T cells in vivo.
Additionally, H2-D1 recombinant proteins contribute to vaccine development and immunotherapy design by elucidating peptide-binding motifs or optimizing antigen presentation. Mutational studies of H2-D1 help map residues critical for peptide selection or T cell recognition, offering insights into MHC evolution and disease susceptibility. Despite its murine origin, H2-D1 serves as a functional analog for human HLA studies, bridging translational research in immunology. Overall, H2-D1 recombinant tools remain indispensable for dissecting MHC-I biology and advancing therapeutic strategies.
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