| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MCM9 |
| Uniprot No | Q9NXL9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-391aa |
| 氨基酸序列 | MNSDQVTLVGQVFESYVSEYHKNDILLILKERDEDAHYPVVVNAMTLFETNMEIGEYFNMFPSEVLTIFDSALRRSALTILQSLSQPEAVSMKQNLHARISGLPVCPELVREHIPKTKDVGHFLSVTGTVIRTSLVKVLEFERDYMCNKCKHVFVIKADFEQYYTFCRPSSCPSLESCDSSKFTCLSGLSSSPTRCRDYQEIKIQEQVQRLSVGSIPRSMKVILEDDLVDSCKSGDDLTIYGIVMQRWKPFQQDVRCEVEIVLKANYIQVNNEQSSGIIMDEEVQKEFEDFWEYYKSDPFAGRNVILASLCPQVFGMYLVKLAVAMVLAGGIQRTDATGTRVRGESHLLLVGDPGTGKSQFLKYAAKITPRSVLTTGIGSTSAGLTVTAVK |
| 预测分子量 | 48.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MCM9重组蛋白的3篇代表性文献(虚构示例，仅供格式参考)：
1. **文献名称**：MCM9 Recombinant Protein Facilitates DNA Repair through Helicase Activity
**作者**：Smith A, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化MCM9重组蛋白，证实其具有ATP依赖性解旋酶活性，并通过体外实验证明MCM9与RAD51协同促进同源重组修复。
2. **文献名称**：Structural Analysis of Human MCM9 Reveals Its Role in Replication Fork Stability
**作者**：Chen L, et al.
**摘要**：利用重组MCM9蛋白进行晶体结构解析，发现其N端结构域与MCM8形成复合物，并通过细胞实验验证其维持复制叉完整性的功能，为卵巢早衰相关突变提供机制解释。
3. **文献名称**：MCM9 Knockout and Recombinant Rescue in Mouse Germ Cells
**作者**：Tanaka K, et al.
**摘要**：构建MCM9基因敲除小鼠模型，通过重组MCM9蛋白回补实验证实其对于减数分裂中双链断裂修复的关键作用，并揭示其与Fanconi贫血通路的功能关联。
注：以上内容为模拟生成，实际文献需通过PubMed/Google Scholar检索关键词“MCM9 recombinant”“MCM8/9 complex”获取，建议结合近期综述(如《DNA Repair》期刊)追踪最新进展。
MCM9 (Minichromosome Maintenance Complex Component 9) is a conserved eukaryotic protein critical for genomic stability, primarily recognized for its dual roles in DNA replication and homologous recombination repair. As a member of the MCM family, it shares structural homology with the MCM2-7 helicase complex but functions distinctly. MCM9 collaborates with MCM8 to form a heterohexameric complex essential for processing stalled replication forks and repairing DNA double-strand breaks (DSBs). This interaction facilitates the loading of RAD51. a key recombinase, onto single-stranded DNA, enabling homologous recombination.
Recombinant MCM9 protein, typically expressed in bacterial (e.g., *E. coli*) or mammalian systems, is engineered to include affinity tags (e.g., His-tag) for purification. Its recombinant form allows biochemical characterization, including helicase activity assays, ATPase kinetics, and protein interaction studies. Research has linked MCM9 dysfunction to reproductive disorders, such as premature ovarian insufficiency, and cancer susceptibility due to impaired DNA repair. Mutant variants identified in clinical studies often show reduced stability or disrupted interaction with MCM8. underscoring its role in genome maintenance.
Recent studies focus on MCM9's involvement in replication stress responses and its potential as a therapeutic target. Structural analyses using recombinant proteins aim to map functional domains and disease-associated mutations. Additionally, MCM9-deficient models highlight its importance in meiosis and embryonic development. Despite progress, mechanistic details of MCM8/9 complex regulation and cross-talk with other repair pathways remain active areas of investigation. Recombinant MCM9 thus serves as a vital tool for dissecting DNA repair mechanisms and developing strategies to address genomic instability-related diseases.
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